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Literature summary extracted from

  • Gavidia, I.; Tarrio, R.; Rodriguez-Trelles, F.; Perez-Bermudez, P.; Ulrich Seitz, H.
    Plant progesterone 5beta-reductase is not homologous to the animal enzyme. Molecular evolutionary characterization of P5betaR from Digitalis purpurea (2007), Phytochemistry, 68, 853-864.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.1.3 additional information P5betaR belongs to the short-chain dehydrogenase/reductase (SDR) superfamily, bearing no structural homology to its mammalian counterpart, which is a member of the aldo-keto reductase (AKR) superfamily Digitalis purpurea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.3 full-length P5betaR cDNA overexpressed in Escherichia coli as a fusion protein with MBP or His-tag Digitalis purpurea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.3 0.0079
-
 NADPH
-
 Digitalis purpurea
1.3.1.3 0.0391
-
 progesterone
-
 Digitalis purpurea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.3 43000
-
 x * 43963, sequence analysis, x * 43000, purified enzyme, SDS–PAGE Digitalis purpurea
1.3.1.3 43963
-
 x * 43963, sequence analysis, x * 43000, purified enzyme, SDS–PAGE Digitalis purpurea

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.3 Digitalis purpurea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.3 to apparent homogeneity by affinity chromatography Digitalis purpurea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.3 flower maximally transcribed in mature flowers Digitalis purpurea
-
1.3.1.3 leaf maximally transcribed Digitalis purpurea
-
1.3.1.3 root
-
 Digitalis purpurea
-
1.3.1.3 stem at the base of the rosette Digitalis purpurea
-
1.3.1.3 vascular bundle at the basal part of the leaf, lowest amount Digitalis purpurea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.3 additional information the recombinant enzyme catalyzes the stereospecific reduction of the delta4-double bond of several steroids with a 3-oxo,delta4,5 structure Digitalis purpurea ?
-
 ?
1.3.1.3 progesterone + NADPH + H+ highest substrate specificity Digitalis purpurea 5beta-pregnane-3,20-dione + NADP+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.3.1.3 ? x * 43963, sequence analysis, x * 43000, purified enzyme, SDS–PAGE Digitalis purpurea

Synonyms

EC Number Synonyms Comment Organism
1.3.1.3 P5betaR
-
 Digitalis purpurea
1.3.1.3 progesterone 5beta-reductase
-
 Digitalis purpurea

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.3 NADPH
-
 Digitalis purpurea