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Literature summary extracted from

  • Miesel, L.; Kravec, C.; Xin, A.T.; McMonagle, P.; Ma, S.; Pichardo, J.; Feld, B.; Barrabee, E.; Palermo, R.
    A high-throughput assay for the adenylation reaction of bacterial DNA ligase (2007), Anal. Biochem., 366, 9-17.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.5.1.2 analysis high-throughput assay for the adenylation reaction of bacterial DNA ligase Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.2
-
Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.2 2-methylthio ADP
-
Escherichia coli
6.5.1.2 2-methylthio-ATP
-
Escherichia coli
6.5.1.2 ADP inhibits adenylation reaction Escherichia coli
6.5.1.2 AMP inhibits adenylation reaction Escherichia coli
6.5.1.2 ATP inhibits adenylation reaction Escherichia coli
6.5.1.2 cAMP inhibits adenylation reaction Escherichia coli
6.5.1.2 Rp-cAMPS
-
Escherichia coli
6.5.1.2 Sp-cAMPS
-
Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.2
-
Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.5.1.2 0.0011
-
37°C, pH 7.4, DNA ligation Escherichia coli 2-methylthio ATP
6.5.1.2 0.0015
-
37°C, pH 7.4, DNA ligation Escherichia coli Sp-3',5'-cyclic adenosine monophosphothiolate
6.5.1.2 0.0041
-
37°C, pH 7.4, DNA ligation Escherichia coli 2-methylthio ADP
6.5.1.2 0.12
-
37°C, pH 7.4, DNA ligation Escherichia coli (Rp)-3',5'-cyclic adenosine monophosphothiolate