EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.1.212 | analysis | a rapid, continuous, and convenient three-enzyme coupled UV absorption assay is developed to quantitate the glucuronic acid and N-acetylglucosamine transferase activities of hyaluronan synthase. Activity is measured by coupling the UDP produced from the PmHAS-catalyzed transfer of UDP-GlcNAc and UDP-GlcUA to a hyaluronic acid tetrasaccharide primer with the oxidation of NADH. Using a fluorescently labeled primer, the products are characterized by gel electrophoresis. The assay can be used to determine kinetic parameters, inhibition constants, and mechanistic aspects of this enzyme. In addition, it can be used to quantify PmHAS during purification of the enzyme from culture media | Pasteurella multocida |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.212 | expression in Escherichia coli | Pasteurella multocida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.212 | additional information | a truncated soluble form of recombinant PmHAS (residues 1703) can catalyze the glycosyl transfers in a time- and concentration-dependent manner | Pasteurella multocida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.212 | 0.014 | - |
UDP-alpha-D-glucuronate | 25°C, pH 7.5 | Pasteurella multocida | |
2.4.1.212 | 0.66 | - |
UDP-alpha-N-acetyl-D-glucosamine | 25°C, pH 7.5 | Pasteurella multocida | |
2.4.1.212 | 0.91 | - |
hyaluronic acid tetrasaccharide | 25°C, pH 7.5 | Pasteurella multocida |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.212 | extracellular | - |
Pasteurella multocida | - |
- |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.212 | Pasteurella multocida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.212 | - |
Pasteurella multocida |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.212 | culture medium | - |
Pasteurella multocida | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.212 | hyaluronic acid tetrasaccharide + UDP-alpha-D-glucuronate | - |
Pasteurella multocida | ? | - |
? | |
2.4.1.212 | hyaluronic acid tetrasaccharide + UDP-alpha-N-acetyl-D-glucosamine | - |
Pasteurella multocida | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.212 | PmHAS | - |
Pasteurella multocida |