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Literature summary extracted from

  • Krupa, J.C.; Shaya, D.; Chi, L.; Linhardt, R.J.; Cygler, M.; Withers, S.G.; Mort, J.S.
    Quantitative continuous assay for hyaluronan synthase (2007), Anal. Biochem., 361, 218-225.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.212 analysis a rapid, continuous, and convenient three-enzyme coupled UV absorption assay is developed to quantitate the glucuronic acid and N-acetylglucosamine transferase activities of hyaluronan synthase. Activity is measured by coupling the UDP produced from the PmHAS-catalyzed transfer of UDP-GlcNAc and UDP-GlcUA to a hyaluronic acid tetrasaccharide primer with the oxidation of NADH. Using a fluorescently labeled primer, the products are characterized by gel electrophoresis. The assay can be used to determine kinetic parameters, inhibition constants, and mechanistic aspects of this enzyme. In addition, it can be used to quantify PmHAS during purification of the enzyme from culture media Pasteurella multocida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.212 expression in Escherichia coli Pasteurella multocida

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.212 additional information a truncated soluble form of recombinant PmHAS (residues 1–703) can catalyze the glycosyl transfers in a time- and concentration-dependent manner Pasteurella multocida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.212 0.014
-
UDP-alpha-D-glucuronate 25°C, pH 7.5 Pasteurella multocida
2.4.1.212 0.66
-
UDP-alpha-N-acetyl-D-glucosamine 25°C, pH 7.5 Pasteurella multocida
2.4.1.212 0.91
-
hyaluronic acid tetrasaccharide 25°C, pH 7.5 Pasteurella multocida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.212 extracellular
-
Pasteurella multocida
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.212 Pasteurella multocida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.212
-
Pasteurella multocida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.212 culture medium
-
Pasteurella multocida
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.212 hyaluronic acid tetrasaccharide + UDP-alpha-D-glucuronate
-
Pasteurella multocida ?
-
?
2.4.1.212 hyaluronic acid tetrasaccharide + UDP-alpha-N-acetyl-D-glucosamine
-
Pasteurella multocida ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.212 PmHAS
-
Pasteurella multocida