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Literature summary extracted from

  • Fernley, R.T.; Iliades, P.; Macreadie, I.
    A rapid assay for dihydropteroate synthase activity suitable for identification of inhibitors (2007), Anal. Biochem., 360, 227-234.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.15 expression in Escherichia coli Saccharomyces cerevisiae
2.7.6.3 expression in Escherichia coli Saccharomyces cerevisiae
2.7.6.3 expression of His6-tagged enzyme in Escherichia coli Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.15 additional information coupled enzymatic spectrophotometric assay to measure the activity of DHPS Saccharomyces cerevisiae
2.5.1.15 P559S mutant with implicated sulfa drug resistance Saccharomyces cerevisiae
2.5.1.15 T557A mutant with implicated sulfa drug resistance Saccharomyces cerevisiae
2.5.1.15 T557A/P559S mutant with implicated sulfa drug resistance Saccharomyces cerevisiae
2.5.1.15 T557V/P559S mutant with implicated sulfa drug resistance Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.15 dapsone
-
 Saccharomyces cerevisiae
2.5.1.15 sulfachloropyridazine
-
 Saccharomyces cerevisiae
2.5.1.15 sulfamethoxazole
-
 Saccharomyces cerevisiae
2.7.6.3 dapsone
-
 Saccharomyces cerevisiae
2.7.6.3 sulfachloropyridazine
-
 Saccharomyces cerevisiae
2.7.6.3 sulfamethoxazole
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.15 0.0034
-
 p-Aminobenzoic acid wild type Saccharomyces cerevisiae
2.5.1.15 0.0485
-
 p-Aminobenzoic acid mutant P559S Saccharomyces cerevisiae
2.5.1.15 0.103
-
 p-Aminobenzoic acid mutant T557A Saccharomyces cerevisiae
2.5.1.15 1.161
-
 p-Aminobenzoic acid mutant T557V/P559S Saccharomyces cerevisiae
2.5.1.15 4.02
-
 p-Aminobenzoic acid mutant T557A/P559S Saccharomyces cerevisiae
2.7.6.3 additional information
-
 additional information kinetics, HPPK-DHPS coupled assay method, overview Saccharomyces cerevisiae
2.7.6.3 0.00039
-
 6-hydroxymethyl-7,8-dihydropterin
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.3 Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate Saccharomyces cerevisiae
-
 diphosphate + 7,8-dihydropteroate
-
 ?
2.7.6.3 6-hydroxymethyl-7,8-dihydropterin + ATP Saccharomyces cerevisiae folate biosynthesis 6-hydroxymethyl-7,8-dihydropterin diphosphate + AMP
-
 ?
2.7.6.3 ATP + 6-hydroxymethyl-7,8-dihydropterin Saccharomyces cerevisiae step in the folate biosynthetic pathway, overview AMP + (7,8-dihydropterin-6-yl)methyl diphosphate
-
 ?
2.7.6.3 additional information Saccharomyces cerevisiae the bifunctional HPPK-DHPS enzyme catalyzes sequential steps in the folate biosynthesis, i.e. the 6-hydroxymethylpterin pyrophosphokinase reaction and the dihydropteroate synthase, DHPS, reaction, EC 2.5.1.15 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.15 Saccharomyces cerevisiae P53848
-
-
2.7.6.3 Saccharomyces cerevisiae P53848
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.3 recombinant His6-tagged enzyme from Escherichia coli by metal affinity chromatography and ion exchange chromatography, cleavage of the tag by thrombin Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate
-
 Saccharomyces cerevisiae diphosphate + 7,8-dihydropteroate
-
 ?
2.7.6.3 6-hydroxymethyl-7,8-dihydropterin + ATP folate biosynthesis Saccharomyces cerevisiae 6-hydroxymethyl-7,8-dihydropterin diphosphate + AMP
-
 ?
2.7.6.3 ATP + 6-hydroxymethyl-7,8-dihydropterin
-
 Saccharomyces cerevisiae AMP + (7,8-dihydropterin-6-yl)methyl diphosphate
-
 ?
2.7.6.3 ATP + 6-hydroxymethyl-7,8-dihydropterin step in the folate biosynthetic pathway, overview Saccharomyces cerevisiae AMP + (7,8-dihydropterin-6-yl)methyl diphosphate
-
 ?
2.7.6.3 additional information the bifunctional HPPK-DHPS enzyme catalyzes sequential steps in the folate biosynthesis, i.e. the 6-hydroxymethylpterin pyrophosphokinase reaction and the dihydropteroate synthase, DHPS, reaction, EC 2.5.1.15 Saccharomyces cerevisiae ?
-
 ?
2.7.6.3 additional information coupled assay method development using reduction of DHPS reaction product dihydropteroate to tetrahydropteroate by excess dihydrofolate reductase with the cofactor NADPH, the oxidation of NADPH is monitored, overview Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.15 7,8-dihydropteroate synthase
-
 Saccharomyces cerevisiae
2.5.1.15 DHPS
-
 Saccharomyces cerevisiae
2.5.1.15 dihydropteroate synthase
-
 Saccharomyces cerevisiae
2.7.6.3 6-hydroxymethylpterin pyrophosphokinase
-
 Saccharomyces cerevisiae
2.7.6.3 HPPK
-
 Saccharomyces cerevisiae
2.7.6.3 HPPK-DHPS
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.3 37
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.3 8.5
-
 assay at Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.15 0.00068
-
 dapsone wild tpye Saccharomyces cerevisiae
2.5.1.15 0.00205
-
 dapsone mutant T557A Saccharomyces cerevisiae
2.5.1.15 0.00295
-
 sulfachloropyridazine wild tpye Saccharomyces cerevisiae
2.5.1.15 0.00371
-
 sulfamethoxazole mutant T557A Saccharomyces cerevisiae
2.5.1.15 0.0063
-
 sulfachloropyridazine mutant T557A Saccharomyces cerevisiae
2.5.1.15 0.0085
-
 sulfamethoxazole wild tpye Saccharomyces cerevisiae
2.5.1.15 0.0148
-
 dapsone mutant P559S Saccharomyces cerevisiae
2.5.1.15 0.0513
-
 sulfachloropyridazine mutant P559S Saccharomyces cerevisiae
2.5.1.15 0.0543
-
 sulfamethoxazole mutant P559S Saccharomyces cerevisiae
2.5.1.15 0.096
-
 sulfachloropyridazine mutant T557A/P559S Saccharomyces cerevisiae
2.5.1.15 0.76
-
 sulfamethoxazole mutant T557V/P559S Saccharomyces cerevisiae
2.5.1.15 0.94
-
 sulfachloropyridazine mutant T557V/P559S Saccharomyces cerevisiae
2.5.1.15 1.16
-
 dapsone mutant T557A/P559S Saccharomyces cerevisiae
2.5.1.15 1.17
-
 sulfamethoxazole mutant T557A/P559S Saccharomyces cerevisiae
2.5.1.15 1.29
-
 dapsone mutant T557V/P559S Saccharomyces cerevisiae