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Literature summary extracted from

  • Luo, L.; Pappalardi, M.B.; Tummino, P.J.; Copeland, R.A.; Fraser, M.E.; Grzyska, P.K.; Hausinger, R.P.
    An assay for Fe(II)/2-oxoglutarate-dependent dioxygenases by enzyme-coupled detection of succinate formation (2006), Anal. Biochem., 353, 69-74.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.17 W98I reduced activity compared to the wild type enzyme Escherichia coli
1.14.11.17 Y73I reduced activity compared to the wild type enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.17 0.009
-
 2-oxoglutarate wild type enzyme, in the presence of taurine Escherichia coli
1.14.11.17 0.012
-
 2-oxoglutarate mutant enzyme Y73I, in the presence of taurine Escherichia coli
1.14.11.17 0.015
-
 2-oxoglutarate mutant enzyme Y73I, without taurine Escherichia coli
1.14.11.17 0.02
-
 2-oxoglutarate wild type enzyme, without taurine Escherichia coli
1.14.11.17 0.048
-
 2-oxoglutarate mutant enzyme W98I, in the presence of taurine Escherichia coli
1.14.11.17 0.052
-
 taurine wild type enzyme Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.17 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.17 taurine + 2-oxoglutarate + O2
-
 Escherichia coli sulfite + aminoacetaldehyde + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.17 Fe(II)/2-oxoglutarate-dependent taurine dioxygenase
-
 Escherichia coli
1.14.11.17 taurine (2-aminoethanesulfonate)/2-oxoglutarate dioxygenase
-
 Escherichia coli
1.14.11.17 taurine/2-oxoglutarate dioxygenase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.11.17 0.06
-
 2-oxoglutarate wild type enzyme, without taurine Escherichia coli
1.14.11.17 0.12
-
 2-oxoglutarate mutant enzyme Y73I, without taurine Escherichia coli
1.14.11.17 0.14
-
 2-oxoglutarate mutant enzyme W98I, in the presence of taurine Escherichia coli
1.14.11.17 1
-
 2-oxoglutarate mutant enzyme Y73I, in the presence of taurine Escherichia coli
1.14.11.17 3
-
 taurine wild type enzyme Escherichia coli
1.14.11.17 3
-
 2-oxoglutarate wild type enzyme, in the presence of taurine Escherichia coli