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Literature summary extracted from

  • Shepherd, M.; Dailey, H.A.
    A continuous fluorimetric assay for protoporphyrinogen oxidase by monitoring porphyrin accumulation (2005), Anal. Biochem., 344, 115-121.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.3.3.4 analysis advantages of the continuous spectrofluorimetric assay over the discontinuous assay is of importance for both the kinetic characterization of recombinant PPOs and the detection of low concentrations of this enzyme in biological samples Mus musculus
1.3.3.4 analysis advantages of the continuous spectrofluorimetric assay over the discontinuous assay is of importance for both the kinetic characterization of recombinant PPOs and the detection of low concentrations of this enzyme in biological samples Myxococcus xanthus
1.3.3.4 analysis advantages of the continuous spectrofluorimetric assay over the discontinuous assay is of importance for both the kinetic characterization of recombinant PPOs and the detection of low concentrations of this enzyme in biological samples Aquifex aeolicus
1.3.3.4 analysis advantages of the continuous spectrofluorimetric assay over the discontinuous assay is of importance for both the kinetic characterization of recombinant PPOs and the detection of low concentrations of this enzyme in biological samples, may be useful for assessing diminished PPO activities in variegate porphyria patient samples Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.4 overexpression in Escherichia coli JM109 cells Homo sapiens
1.3.3.4 overexpression in Escherichia coli JM109 cells Myxococcus xanthus
1.3.3.4 overexpression in Escherichia coli JM109 cells Aquifex aeolicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.4 acifluorfen
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.4 0.001
-
 protoporphyrinogen-IX
-
 Aquifex aeolicus
1.3.3.4 0.0036
-
 protoporphyrinogen-IX
-
 Myxococcus xanthus
1.3.3.4 0.0038
-
 protoporphyrinogen-IX
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.3.4 mitochondrion
-
 Mus musculus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.4 Aquifex aeolicus
-
-
-
1.3.3.4 Homo sapiens
-
-
-
1.3.3.4 Mus musculus
-
-
-
1.3.3.4 Myxococcus xanthus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.4 by gel filtration Homo sapiens
1.3.3.4 by gel filtration Myxococcus xanthus
1.3.3.4 by gel filtration Aquifex aeolicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.3.4 liver
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.4 0.0000007167
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.4 protoporphyrinogen-IX + 3 O2
-
 Mus musculus protoporphyrin-IX + 3 H2O2
-
 ?
1.3.3.4 protoporphyrinogen-IX + 3 O2
-
 Homo sapiens protoporphyrin-IX + 3 H2O2
-
 ?
1.3.3.4 protoporphyrinogen-IX + 3 O2
-
 Myxococcus xanthus protoporphyrin-IX + 3 H2O2
-
 ?
1.3.3.4 protoporphyrinogen-IX + 3 O2
-
 Aquifex aeolicus protoporphyrin-IX + 3 H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.3.4 PPO
-
 Mus musculus
1.3.3.4 PPO
-
 Homo sapiens
1.3.3.4 PPO
-
 Myxococcus xanthus
1.3.3.4 PPO
-
 Aquifex aeolicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.3.4 0.00053
-
 acifluorfen
-
 Homo sapiens