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Literature summary extracted from
- Fundamental differences in bioaffinity of amino acid dehydrogenases for N6- and S6-linked immobilized cofactors using kinetic-based enzyme-capture strategies (2005), Anal. Biochem., 338, 102-112.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.1 | D-Cys | - |
Bacillus subtilis |  |
| 1.4.1.3 | Glutarate | shows no affinity for N6-linked NAD+ but is biospecifically adsorbed to S6-linked NAD+ derivatives in the presence of its soluble kinetic-based enzyme capture ligand glutarate | Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.1.1 | 1.63 | - |
L-Ala | at pH 8.6 | Bacillus subtilis | |
| 1.4.1.1 | 1.96 | - |
L-Ala | at pH 7.4 | Bacillus subtilis | |
| 1.4.1.3 | 0.24 | - |
L-glutamate | at pH 8.5 | Bos taurus | |
| 1.4.1.3 | 1.01 | - |
L-glutamate | at pH 7.4 | Bos taurus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.1.1 | Bacillus subtilis | - |
- |
- |
| 1.4.1.3 | Bos taurus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.4.1.3 | liver | - |
Bos taurus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Bacillus subtilis | pyruvate + NH3 + NADH | - |
? | |
| 1.4.1.3 | 2-oxoglutarate + NAD(P)H + NH3 | - |
Bos taurus | L-glutamate + NAD(P)+ + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.4.1.1 | ALADH | - |
Bacillus subtilis |
| 1.4.1.1 | NAD+-dependent alanine dehydrogenase | - |
Bacillus subtilis |
| 1.4.1.3 | GDH | - |
Bos taurus |
| 1.4.1.3 | NAD(P)+-dependent glutamate dehydrogenase | - |
Bos taurus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.3 | 7.4 | 8.5 | - |
Bos taurus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.1 | NAD+ | affinity for NAD+ immobilized through an N6 linkage, as opposed to an N1 linkage, replacement of the nitrogen with sulfur to produce an S6 linkage | Bacillus subtilis | |
| 1.4.1.3 | NAD(P)H | - |
Bos taurus | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.1.1 | 0.71 | - |
D-Cys | at pH 8.6 | Bacillus subtilis | |
| 1.4.1.1 | 1.88 | - |
D-Cys | at pH 7.4 | Bacillus subtilis | |
| 1.4.1.3 | 0.07 | - |
Glutarate | at pH 8.5 | Bos taurus | |
| 1.4.1.3 | 0.09 | - |
Glutarate | at pH 7.4 | Bos taurus | |
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Release 2023.1 (January 2023)
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