Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Saito, M.; Nishimura, K.; Wakabayashi, S.; Kurihara, T.; Nagata, Y.
    Purification of branched-chain amino acid aminotransferase from Helicobacter pylori NCTC 11637 (2007), Amino Acids, 33, 445-449.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.42 0.085
-
 2-oxoglutarate pH 8.0, 37°C Helicobacter pylori
2.6.1.42 0.085
-
 2-oxoglutarate in 50mM Tris-HCl buffer, pH 8.0, at 37°C Helicobacter pylori
2.6.1.42 0.34
-
 L-isoleucine pH 8.0, 37°C Helicobacter pylori
2.6.1.42 0.34
-
 L-isoleucine in 50mM Tris-HCl buffer, pH 8.0, at 37°C Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.42 36000
-
 2 * 37539, calculated, 2 * 36000, SDS-PAGE Helicobacter pylori
2.6.1.42 37539
-
 2 * 37539, calculated, 2 * 36000, SDS-PAGE Helicobacter pylori
2.6.1.42 37540
-
 calculated from sequence of cDNA Helicobacter pylori
2.6.1.42 68500
-
 gel filtration Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Helicobacter pylori
-
-
-
2.6.1.42 Helicobacter pylori Q0E9S8
-
-
2.6.1.42 Helicobacter pylori NCTC 11637
-
-
-
2.6.1.42 Helicobacter pylori NCTC 11637 Q0E9S8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42 DEAE Toyopearl column chromatography, Phenyl Sepharose column chromatography, DEAE Sepharose column chromatography, Shodex KW-2003 gel filtration, and Butyl Toyopearl gel filtration Helicobacter pylori

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 glycine + 2-oxoglutarate
-
 Helicobacter pylori glyoxylate + L-glutamate 0.4% of the activity with L-isoleucine ?
2.6.1.42 L-aspartate + 2-oxoglutarate
-
 Helicobacter pylori oxaloacetate + L-glutamate 1.7% of the activity with L-isoleucine ?
2.6.1.42 L-aspartate + 2-oxoglutarate
-
 Helicobacter pylori NCTC 11637 oxaloacetate + L-glutamate 1.7% of the activity with L-isoleucine ?
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Helicobacter pylori 3-methyl-2-oxopentanoate + L-glutamate
-
 ?
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Helicobacter pylori NCTC 11637 3-methyl-2-oxopentanoate + L-glutamate
-
 ?
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Helicobacter pylori 4-methyl-2-oxopentanoate + L-glutamate
-
 ?
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Helicobacter pylori 4-methyl-2-oxopentanoate + L-glutamate 70.1% of the activity with L-isoleucine ?
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Helicobacter pylori NCTC 11637 4-methyl-2-oxopentanoate + L-glutamate
-
 ?
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Helicobacter pylori NCTC 11637 4-methyl-2-oxopentanoate + L-glutamate 70.1% of the activity with L-isoleucine ?
2.6.1.42 L-methionine + 2-oxoglutarate
-
 Helicobacter pylori 4-methylsulfanyl-2-oxobutanoate + L-glutamate 2.9% of the activity with L-isoleucine ?
2.6.1.42 L-methionine + 2-oxoglutarate
-
 Helicobacter pylori NCTC 11637 4-methylsulfanyl-2-oxobutanoate + L-glutamate 2.9% of the activity with L-isoleucine ?
2.6.1.42 L-phenylalanine + 2-oxoglutarate
-
 Helicobacter pylori phenylpyruvate + L-glutamate 0.9% of the activity with L-isoleucine ?
2.6.1.42 L-phenylalanine + 2-oxoglutarate
-
 Helicobacter pylori NCTC 11637 phenylpyruvate + L-glutamate 0.9% of the activity with L-isoleucine ?
2.6.1.42 L-valine + 2-oxoglutarate
-
 Helicobacter pylori 3-methyl-2-oxobutanoate + L-glutamate
-
 ?
2.6.1.42 L-valine + 2-oxoglutarate
-
 Helicobacter pylori 3-methyl-2-oxobutanoate + L-glutamate 47.9% of the activity with L-isoleucine ?
2.6.1.42 L-valine + 2-oxoglutarate
-
 Helicobacter pylori NCTC 11637 3-methyl-2-oxobutanoate + L-glutamate
-
 ?
2.6.1.42 additional information no or very weak activity is shown toward L-methionine, L-aspartate, L-phenylalanine, glycine and the D-enantiomers of leucine, isoleucine and valine Helicobacter pylori ?
-
 ?
2.6.1.42 additional information no substrate: L-asparagine, L-histidine, and L-lysine, D-isoleucine, D-leucine, D-valine, as well as both enantiomers of alanine, proline, and serine Helicobacter pylori ?
-
 ?
2.6.1.42 additional information no or very weak activity is shown toward L-methionine, L-aspartate, L-phenylalanine, glycine and the D-enantiomers of leucine, isoleucine and valine Helicobacter pylori NCTC 11637 ?
-
 ?
2.6.1.42 additional information no substrate: L-asparagine, L-histidine, and L-lysine, D-isoleucine, D-leucine, D-valine, as well as both enantiomers of alanine, proline, and serine Helicobacter pylori NCTC 11637 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.42 dimer 2 * 37539, calculated, 2 * 36000, SDS-PAGE Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 BcaT
-
 Helicobacter pylori
2.6.1.42 branched-chain amino acid aminotransferase
-
 Helicobacter pylori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.42 37
-
-
 Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.42 8
-
-
 Helicobacter pylori

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.42 pyridoxal 5'-phosphate
-
 Helicobacter pylori

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.6.1.42 Helicobacter pylori calculated from sequence of cDNA
-
 6.47
2.6.1.42 Helicobacter pylori calculated
-
 6.5