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Literature summary extracted from

  • Zhang, W.; Xia, X.; Jalal, D.I.; Kuncewicz, T.; Xu, W.; Lesage, G.D.; Kone, B.C.
    Aldosterone-sensitive repression of ENaCalpha transcription by a histone H3 lysine-79 methyltransferase (2006), Am. J. Physiol. Cell Physiol., 290, C936-C946.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.1.1.360 additional information mDot1a is a novel aldosterone regulated histone modification enzyme, and, through binding the ENaCalpha promoter and hypermethylating histone H3 K79 associated with the ENaCalpha promoter, a negative regulator of ENaCalpha transcription Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.360 into vector pcDNA3.1 (+) Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.360 additional information methyltransferase-dead mutant pEGFP-mDot1aRCR, mENaCalpha mRNA levels in cells transfected with the methyltransferase-dead mutant are almost twice that of vector-transfected cells, knockdown of mDot1a mRNA levels by RNA interference augments endogenous mENaCalpha expression and activity of mENaCalpha-promoter luciferase reporter Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.360 aldosterone
-
 Homo sapiens
2.1.1.360 aldosterone at an early time point (2 h) downregulates the steady-state level of mDot1a mRNA and this precedes, and is associated with, downregulation of histone H3 K79 methylation in mIMCD3 cells Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.360 170000
-
 Western blot analysis, mDot1a in the kidney Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.360 Homo sapiens
-
-
-
2.1.1.360 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.360 epithelial cell
-
 Mus musculus
-
2.1.1.360 HEK-293T cell
-
 Homo sapiens
-
2.1.1.360 kidney
-
 Mus musculus
-
2.1.1.360 additional information mIMCD3 cell, mDot1a expressed and maintained at low levels Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.360 additional information mDot1a directly or indirectly associates with and hypermethylated histone H3 K79 at specific portions of the ENaCalpha 5'-flanking region, inducing a local alteration of the chromatin structure that facilitates ENaCalpha mRNA expression Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.360 histone H3 lysine-79 methyltransferase
-
 Mus musculus
2.1.1.360 histone H3 lysine-79 methyltransferase
-
 Homo sapiens
2.1.1.360 mDot1a
-
 Mus musculus
2.1.1.360 mDot1a
-
 Homo sapiens
2.1.1.360 murine disruptor of telomeric silencing alternative splice variant a
-
 Mus musculus
2.1.1.360 murine disruptor of telomeric silencing alternative splice variant a
-
 Homo sapiens