Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bocola, M.; Schulz, F.; Leca, F.; Vogel, A.; Fraaije, M.W.; Reetz, M.T.
    Converting phenylacetone monooxygenase into phenylcyclohexanone monooxygenase by rational design: towards practical Baeyer-Villiger monooxygenases (2005), Adv. Synth. Catal., 347, 979-986.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.92 PAMO crystal structure analysis, comparison to cyclohexanone monooxygenase, EC 1.14.13.22 Thermobifida fusca

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.92 additional information construction of three mutants P1-P3 by elimination of a bulge loop region, involving residues Ser441, Ala442, and Leu443, leading to enhanced substrate enantioselectivity of Baeyer-Villiger reactions while maintaining high thermal stability, overview Thermobifida fusca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.92 0.059
-
 phenylacetone wild-type enzyme Thermobifida fusca
1.14.13.92 0.07
-
 2-phenylcyclohexanone mutant P3 Thermobifida fusca
1.14.13.92 0.5
-
 2-phenylcyclohexanone mutant P2 Thermobifida fusca
1.14.13.92 2.3
-
 2-phenylcyclohexanone mutant P1 Thermobifida fusca
1.14.13.92 2.5
-
 phenylacetone mutant P3 Thermobifida fusca
1.14.13.92 3
-
 phenylacetone mutant P1 Thermobifida fusca
1.14.13.92 4
-
 phenylacetone mutant P2 Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.92 Thermobifida fusca
-
 a moderately thermophilic bacterium
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.13.92 phenylacetone + NADPH + H+ + O2 = benzyl acetate + NADP+ + H2O reaction mechanism Thermobifida fusca
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.92 additional information
-
-
 Thermobifida fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.92 2-methylphenylcyclohexanone + NADPH + O2 mutant P3 prefers the R-isomer Thermobifida fusca 7-benzyloxepan-2-one + NADP+ + H2O
-
 ?
1.14.13.92 2-phenylcyclohexanone + NADPH + O2 molecular modeling of the Criegee intermediate, the wild-type enzyme prefers the S-isomer, while mutants P1-P3 all prefer the R-isomer Thermobifida fusca 7-phenyloxepan-2-one + NADP+ + H2O
-
 ?
1.14.13.92 4-phenylcyclohexanone + NADPH + O2
-
 Thermobifida fusca 4-phenyl-hexano-6-lactone + NADP+ + H2O
-
 ?
1.14.13.92 additional information substrate selectivity and stereospecificity of wild-type and mutant enzymes, overview Thermobifida fusca ?
-
 ?
1.14.13.92 phenylacetone + NADPH + H+ + O2
-
 Thermobifida fusca benzyl acetate + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.92 More structure analysis, the enzyme exhibits a two-domain architecture, and a bulge loop region Thermobifida fusca

Synonyms

EC Number Synonyms Comment Organism
1.14.13.92 More the enzyme belongs to the Baeyer-Villiger monooxygenases Thermobifida fusca
1.14.13.92 PAMO
-
 Thermobifida fusca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.92 25
-
 assay at Thermobifida fusca

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.92 0.22
-
 phenylacetone mutant P3 Thermobifida fusca
1.14.13.92 0.25
-
 phenylacetone mutant P1 Thermobifida fusca
1.14.13.92 0.25
-
 2-phenylcyclohexanone mutant P3 Thermobifida fusca
1.14.13.92 0.31
-
 2-phenylcyclohexanone mutant P1 Thermobifida fusca
1.14.13.92 0.4
-
 phenylacetone mutant P2 Thermobifida fusca
1.14.13.92 0.5
-
 2-phenylcyclohexanone mutant P2 Thermobifida fusca
1.14.13.92 1.9
-
 phenylacetone wild-type enzyme Thermobifida fusca

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.92 FAD binding structure involving Arg337, overview Thermobifida fusca
1.14.13.92 NADPH
-
 Thermobifida fusca