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Literature summary extracted from
- Development of efficient xylose fermentation in Saccharomyces cerevisiae: xylose isomerase as a key component (2007), Adv. Biochem. Eng. Biotechnol., 108, 179-204.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 5.3.1.5 | energy production | engineering of Saccharomyces cerevisiae for alcoholic fermentation of D-xylose | Thermus thermophilus |
| 5.3.1.5 | energy production | engineering Saccharomyces cerevisiae for alcoholic fermentation of D-xylose | Piromyces sp. |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.5 | Saccharomyces cerevisiae is transformed with a gene encoding xylose isomerase (Thermus thermophilus) | Thermus thermophilus |
| 5.3.1.5 | Saccharomyces cerevisiae is transformed with a gene encoding xylose isomerase originating from Piromyces sp. | Piromyces sp. |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.3.1.5 | additional information | Saccharomyces cerevisiae: five enzymes of non-oxidative pentose-phosphate-pathway are induced, a non-specific aldose reductase is deleted | Thermus thermophilus |
| 5.3.1.5 | additional information | Saccharomyces cerevisiae: five enzymes of non-oxidative pentose-phosphate-pathway are induced, a unspecific aldose reductase is deleted | Piromyces sp. |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.5 | Co2+ | depends on the origin of the enzyme | Thermus thermophilus |  |
| 5.3.1.5 | Co2+ | depends on the origin of the enzyme | Piromyces sp. | |
| 5.3.1.5 | Mg2+ | depends on the origin of the enzyme | Thermus thermophilus | |
| 5.3.1.5 | Mg2+ | depends on the origin of the enzyme | Piromyces sp. | |
| 5.3.1.5 | Mn2+ | depends on the origin of the enzyme | Thermus thermophilus | |
| 5.3.1.5 | Mn2+ | depends on the origin of the enzyme | Piromyces sp. | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.5 | D-Xylose | Thermus thermophilus | - |
D-Xylulose | - |
r | |
| 5.3.1.5 | D-Xylose | Piromyces sp. | - |
D-Xylulose | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.5 | no activity in Saccharomyces cerevisiae | - |
- |
- |
| 5.3.1.5 | Piromyces sp. | - |
source of xylose isomerase gene, a phylogenetic tree of the diversity of xylose isomerase genes is given - based on GenBank database | - |
| 5.3.1.5 | Thermus thermophilus | - |
source of xylose isomerase gene, a phylogenetic tree of the diversity of xylose isomerase genes is given - based on GenBank database | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.5 | additional information | - |
measurement of the degradation of xylose and glucose | Thermus thermophilus |
| 5.3.1.5 | additional information | - |
measurement of the degradation of xylose and glucose | Piromyces sp. |
| 5.3.1.5 | additional information | - |
measurement of the production of ethanol, glycerol, organic acids, xylitol, CO2 | Thermus thermophilus |
| 5.3.1.5 | additional information | - |
measurement of the production of ethanol, glycerol, organic acids, xylitol, CO2 | Piromyces sp. |
| 5.3.1.5 | additional information | - |
strain is cultivated under aerobic and anaerobic conditions | Thermus thermophilus |
| 5.3.1.5 | additional information | - |
strains are cultivated under aerobic and anaerobic conditions | Piromyces sp. |
| 5.3.1.5 | 0.017 | - |
Saccharomyces cerevisiae overexprssing five own enzymes of pentose phosphate pathway, deletion of an unspecific aldose reductase, overexpressing xylose isomerase from Piromyces sp., 30°C | Piromyces sp. |
| 5.3.1.5 | 0.04 | - |
Saccharomyces cerevisiae overexpressing xylose isomerase from Thermus thermophilus, 30°C | Thermus thermophilus |
| 5.3.1.5 | 1 | - |
Saccharomyces cerevisiae overexpressing xylose isomerase from Thermus thermophilus, 85°C | Thermus thermophilus |
| 5.3.1.5 | 1.1 | - |
Saccharomyces cerevisiae overexprssing xylose isomerase from Piromyces sp., 30°C | Piromyces sp. |
| 5.3.1.5 | 1.1 | - |
Saccharomyces cerevisiae strain CEN.PK overexprssing xylose isomerase (xylA gene) from Piromyces sp., TPI1 promoter, 30°C | Piromyces sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.5 | D-Glucose | - |
Thermus thermophilus | D-Fructose | - |
r | |
| 5.3.1.5 | D-Glucose | - |
Piromyces sp. | D-Fructose | - |
r | |
| 5.3.1.5 | D-Xylose | - |
Thermus thermophilus | D-Xylulose | - |
r | |
| 5.3.1.5 | D-Xylose | - |
Piromyces sp. | D-Xylulose | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.1.5 | D-xylose ketol isomerase | - |
Thermus thermophilus |
| 5.3.1.5 | D-xylose ketol isomerase | - |
Piromyces sp. |
| 5.3.1.5 | xylose isomerase | - |
Thermus thermophilus |
| 5.3.1.5 | xylose isomerase | - |
no activity in Saccharomyces cerevisiae |
| 5.3.1.5 | xylose isomerase | - |
Piromyces sp. |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.5 | 60 | 80 | - |
Thermus thermophilus |
| 5.3.1.5 | 60 | 80 | - |
Piromyces sp. |
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Release 2023.1 (January 2023)
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