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Literature summary extracted from

  • Newman, J.A.; Das, S.K.; Sedelnikova, S.E.; Rice, D.W.
    Cloning, purification and preliminary crystallographic analysis of a putative pyridoxal kinase from Bacillus subtilis (2006), Acta Crystallogr. Sect. F, F62, 1006-1009.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.35 expressed in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.35 hanging drop vapour diffusion method using 28% PEG 4000, 0.17 M sodium acetate trihydrate, and 0.1 M Tris-HCl (pH 8.5) Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.35 Mg2+
-
 Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.35 40000
-
 SDS-PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.35 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.35 Phenyl ToyoPearl 650S column chromatography and Hi-Load Superdex 200 column gel filtration Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.35 ATP + pyridoxal
-
 Bacillus subtilis ADP + pyridoxal 5'-phosphate
-
 ir
2.7.1.35 ATP + pyridoxamine
-
 Bacillus subtilis ADP + pyridoxamine 5'-phosphate
-
 ir
2.7.1.35 ATP + pyridoxine
-
 Bacillus subtilis ADP + pyridoxine 5'-phosphate
-
 ir
2.7.1.35 additional information the enzyme possesses also a 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase activity Bacillus subtilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.35 hexamer trimer or hexamer, X-ray crystallography Bacillus subtilis
2.7.1.35 trimer trimer or hexamer, X-ray crystallography Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.35 PdxK
-
 Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.35 ATP
-
 Bacillus subtilis