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Literature summary extracted from

  • Young, P.G.; Smith, C.A.; Sun, X.; Baker, E.N.; Metcalf, P.
    Purification, crystallization and preliminary X-ray analysis of Mycobacterium tuberculosis folylpolyglutamate synthase (MtbFPGS) (2006), Acta Crystallogr. Sect. F, 62, 579-582.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.17 expressed in Escherichia coli strain BL21 (DELTADE3) Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.2.17 batch method in the presence ADP and CoCl2 or vapour diffusion in the presence of ADP, dihydrofolate and CaCl2 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.17 50900
-
 predicted from amino acid sequence Mycobacterium tuberculosis
6.3.2.17 51000
-
 SDS-PAGE Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.17 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.17 Ni-Sepharose 6 Fastflow resin chromatography and Superdex-200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.17 ATP + (6RS)-5,6,7,8-tetrahydrofolic acid + L-glutamate
-
 Mycobacterium tuberculosis ADP + phosphate + (6S)-5,6,7,8-tetrahydrofolyl-Glu
-
 ir

Subunits

EC Number Subunits Comment Organism
6.3.2.17 monomer 1 * 51000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
6.3.2.17 Folylpolyglutamate synthase
-
 Mycobacterium tuberculosis
6.3.2.17 FPGS
-
 Mycobacterium tuberculosis