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Literature summary extracted from

  • Lee, K.; Friemann, R.; Parales, J.V.; Gibson, D.T.; Ramaswamy, S.
    Purification, crystallization and preliminary X-ray diffraction studies of the three components of the toluene 2,3-dioxygenase enzyme system (2005), Acta crystallogr. Sect. F, 61, 669-672.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.11 expressed in Escherichia coli strain CGSC7692 (pDTG601A) Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.12.11 hanging drop and sitting drop vapour diffusion method using 40% (w/v) polyethylene glycol 600, 0.1 M sodium citrate pH 6.1 and 20-50 mM Fe(NH4)2(SO4) Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.11 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.11 Q-Sepharose FF column chromatography Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.11 toluene + NADH + O2
-
 Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.11 oxygenaseTOL
-
 Pseudomonas putida
1.14.12.11 TDO
-
 Pseudomonas putida
1.14.12.11 toluene 2,3-dioxygenase
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.11 NADH
-
 Pseudomonas putida