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Literature summary extracted from

  • Das, S.; Kumar, P.; Bhor, V.; Surolia, A.; Vijayan, M.
    Invariance and variability in bacterial PanK: a study based on the crystal structure of Mycobacterium tuberculosis PanK (2006), Acta Crystallogr. Sect. D, 62, 628-638.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.33 expressed in Escherichia coli BL21 (DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.33 hanging drop vapour diffusion method using 10-15% (w/v) PEG 8000, 0.05-0.1 M NaOAc and 0.05 M NaCl dissolved in 0.1 M sodium cacodylate buffer pH 6.5 Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.33 CoA feedback inhibitor Escherichia coli
2.7.1.33 CoA feedback inhibitor Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.33 36000
-
 2 * 36000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Escherichia coli P0A6I3
-
-
2.7.1.33 Mycobacterium tuberculosis P9WPA7
-
-
2.7.1.33 Mycobacterium tuberculosis H37Rv P9WPA7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.33 Ni-NTA metal affinity column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate
-
 Mycobacterium tuberculosis ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate
-
 Escherichia coli ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate
-
 Mycobacterium tuberculosis H37Rv ADP + (R)-4'-phosphopantothenate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.33 homodimer 2 * 36000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.1.33 PanK
-
 Mycobacterium tuberculosis
2.7.1.33 PanK
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.33 ATP
-
 Mycobacterium tuberculosis
2.7.1.33 ATP
-
 Escherichia coli