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Literature summary extracted from

  • Hurt, D.E.; Widom, J.; Clardy, J.
    Structure of Plasmodium falciparum dihydroorotate dehydrogenase with a bound inhibitor (2006), Acta Crystallogr. Sect. D, 62, 312-323.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.98.1 additional information species-specific preferential inhibitor binding, different binding mode for the same inhibitor in the two catalytically identical enzymes human DHODH and Plasmodium DHODH Plasmodium falciparum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.98.1 into vector pRSET and expressed in either freshly transformed or glycerol stocked Escherichia coli BL21-CodonPlus (DE3)-RIL Plasmodium falciparum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.98.1 by sitting-drop vapor-diffusion technique, to 2.4 A resolution Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.98.1 additional information brequinar and DPC-AE661100 have no effect, even at a concentration of 1 mM Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.98.1 0.0131
-
 2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone at saturating concentration of dihydroorotate Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.98.1 50000
-
 gel filtration Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
1.3.98.1 Plasmodium falciparum Q08210 synthetic construct
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.98.1 soniciation and gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.98.1 dihydroorotate + 2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone
-
 Plasmodium falciparum orotate + reduced 2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone
-
 ?
1.3.98.1 dihydroorotate + 2,6-dichloroindophenol
-
 Plasmodium falciparum orotate + reduced 2,6-dichloroindophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.98.1 DHODH
-
 Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.98.1 additional information
-
 2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone at saturating concentration of dihydroorotate Plasmodium falciparum
1.3.99.11 6.15
-
 2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone at saturating concentration of dihydroorotate Plasmodium falciparum