BRENDA - Enzyme Database show

The structure of human deoxycytidine kinase in complex with clofarabine reveals key interactions for prodrug activation

Zhang, Y.; Secrist, J.A.; Ealick, S.E.; Acta Crystallogr. Sect. D 62, 133-139 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.74
expressed as His-tag fusion protein in Escherichia coli BL21(DE3)
Homo sapiens
Crystallization (Commentary)
EC Number
Crystallization
Organism
2.7.1.74
hanging drop vapour diffusion method, in complex with 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine and ADP
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
NTP + 2'-deoxyadenosine
Homo sapiens
-
NDP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.74
NTP + 2'-deoxycytidine
Homo sapiens
more effective substrate than 2'-deoxyadenosine and 2'-deoxyguanosine
NDP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
NTP + 2'-deoxyguanosine
Homo sapiens
-
NDP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.74
NTP + 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine
Homo sapiens
i.e. clofarabine, rate-limiting step in the activation of the prodrug, precursor of an effective chemotherapeutic agent for leukemias and other haematological malignancies
NDP + 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine 5'-phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
P27707
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.74
recombinant protein using His-tag
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
NTP + 2'-deoxyadenosine
-
671043
Homo sapiens
NDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + 2'-deoxycytidine
more effective substrate than 2'-deoxyadenosine and 2'-deoxyguanosine
671043
Homo sapiens
NDP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + 2'-deoxyguanosine
-
671043
Homo sapiens
NDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine
i.e. clofarabine, rate-limiting step in the activation of the prodrug, precursor of an effective chemotherapeutic agent for leukemias and other haematological malignancies
671043
Homo sapiens
NDP + 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine 5'-phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.74
dimer
crystal structure analysis
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
expressed as His-tag fusion protein in Escherichia coli BL21(DE3)
Homo sapiens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.7.1.74
hanging drop vapour diffusion method, in complex with 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine and ADP
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
NTP + 2'-deoxyadenosine
Homo sapiens
-
NDP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.74
NTP + 2'-deoxycytidine
Homo sapiens
more effective substrate than 2'-deoxyadenosine and 2'-deoxyguanosine
NDP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
NTP + 2'-deoxyguanosine
Homo sapiens
-
NDP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.74
NTP + 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine
Homo sapiens
i.e. clofarabine, rate-limiting step in the activation of the prodrug, precursor of an effective chemotherapeutic agent for leukemias and other haematological malignancies
NDP + 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine 5'-phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
recombinant protein using His-tag
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
NTP + 2'-deoxyadenosine
-
671043
Homo sapiens
NDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + 2'-deoxycytidine
more effective substrate than 2'-deoxyadenosine and 2'-deoxyguanosine
671043
Homo sapiens
NDP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + 2'-deoxyguanosine
-
671043
Homo sapiens
NDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine
i.e. clofarabine, rate-limiting step in the activation of the prodrug, precursor of an effective chemotherapeutic agent for leukemias and other haematological malignancies
671043
Homo sapiens
NDP + 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine 5'-phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.74
dimer
crystal structure analysis
Homo sapiens