Literature summary extracted from
Someya, Y.; Takeda, N.; Miyamura, T.
Characterization of the norovirus 3C-like protease (2005), Virus Res., 110, 91-97.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.4.22.66 |
IPTG |
induction of gene expression |
Chiba virus |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.4.22.66 |
expression in Escherichia coli BL21 (DE3) |
Chiba virus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.4.22.66 |
C139A |
cysteine residue responsible for hydrolysis |
Chiba virus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.4.22.66 |
2-mercaptoethanol |
20% inhibited by 0.01 mM, 40% inhibited by 5 mM |
Chiba virus |
|
3.4.22.66 |
Hg2+ |
5 mM completely inhibits |
Chiba virus |
|
3.4.22.66 |
K+ |
inhibited by 100 mM |
Chiba virus |
|
3.4.22.66 |
methyl methanethiosulfonate |
almost complete inhibition |
Chiba virus |
|
3.4.22.66 |
additional information |
not inhibited by Mg2+, Ca2+, 0.01 mM iodoacetate nor 0.01 mM iodoacetamide |
Chiba virus |
|
3.4.22.66 |
N-ethylmaleimide |
almost complete inhibition |
Chiba virus |
|
3.4.22.66 |
N-Phenylmaleimide |
almost complete inhibition |
Chiba virus |
|
3.4.22.66 |
Na+ |
83% inhibited by 100 mM |
Chiba virus |
|
3.4.22.66 |
p-chloromercuribenzenesulfonate |
27% inhibited by 0.01 mM |
Chiba virus |
|
3.4.22.66 |
p-chloromercuribenzoic acid |
82% inhibited by 0.01 mM |
Chiba virus |
|
3.4.22.66 |
phenyl methylsulfonylfluoride |
20% inhibition by 0.01 mM |
Chiba virus |
|
3.4.22.66 |
Zn2+ |
5 mM completely inhibits |
Chiba virus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.4.22.66 |
20600 |
- |
calculated from ProHis protein |
Chiba virus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.22.66 |
Chiba virus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.4.22.66 |
- |
Chiba virus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.4.22.66 |
His3Cd-GST-protein + H2O |
- |
Chiba virus |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.22.66 |
3C-like protease |
- |
Chiba virus |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.4.22.66 |
37 |
- |
80% enzyme activity |
Chiba virus |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
3.4.22.66 |
4 |
48 |
- |
Chiba virus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.4.22.66 |
8.62 |
- |
100% enzyme activity |
Chiba virus |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.4.22.66 |
7.22 |
9.08 |
- |
Chiba virus |