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Literature summary extracted from
- The RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activities of Dengue virus protein NS3 are Mg2+-dependent and require a functional Walker B motif in the helicase catalytic core (2004), Virology, 328, 208-218.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.15 | DTT | - |
Dengue virus |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.6.1.15 | expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Dengue virus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.6.1.15 | D284A | site-directed mutagenesis, mutation in the Walker B motif, inactive mutant | Dengue virus |
| 3.6.1.15 | E285A | site-directed mutagenesis, mutation in the Walker B motif, the mutant does not show RTPase and NTPase activities, and is unable to remove gamma-phosphate from substrate pppAC5 | Dengue virus |
| 3.6.1.15 | E578A | site-directed mutagenesis, mutation of a highly conserved residue, the mutant retains full NTPase, RTPase and helicase activities | Dengue virus |
| 3.6.1.15 | K199A | site-directed mutagenesis, mutation in the Walker B motif, the mutant does not show RTPase and NTPase activities, and is unable to remove gamma-phosphate from substrate pppAC5 | Dengue virus |
| 3.6.1.15 | additional information | construction of mutant DELTANS3 comprising the C-terminal 54-kDa domain of NS3, amino acid residues 169-619 | Dengue virus |
| 3.6.1.15 | R513A | site-directed mutagenesis, mutation in the C-terminal domain of DELTANS3, the mutation reduces NTPase activity, abrogates helicase activity and strongly diminishes RTPase activity | Dengue virus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.15 | additional information | - |
additional information | kinetics | Dengue virus | |
| 3.6.1.15 | 0.108 | - |
ATP | recombinant wild-type enzyme, pH 7.4, 30°C | Dengue virus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.15 | Mg2+ | strictly dependent on, coordination of Mg2+ by the Walker B motif, required for all three activities, the RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activity | Dengue virus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.15 | ATP + H2O | Dengue virus | ATPase activity | ADP + phosphate | - |
? | |
| 3.6.1.15 | additional information | Dengue virus | the enzyme is involved in RNA replication and capping, overview | ? | - |
? | |
| 3.6.1.15 | NTP + H2O | Dengue virus | NTPase activity | NDP + phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.1.15 | Dengue virus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.6.1.15 | recombinat His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Dengue virus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.15 | additional information | - |
the RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activities of wild-type andmutant enzymes, overview | Dengue virus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.15 | ATP + H2O | ATPase activity | Dengue virus | ADP + phosphate | - |
? | |
| 3.6.1.15 | additional information | the enzyme is involved in RNA replication and capping, overview | Dengue virus | ? | - |
? | |
| 3.6.1.15 | additional information | the multifunctional enzyme shows RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activities, which are located on the C-terminal 54-kDa domain, overview | Dengue virus | ? | - |
? | |
| 3.6.1.15 | NTP + H2O | NTPase activity | Dengue virus | NDP + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.6.1.15 | More | RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activities are located on the C-terminal 54-kDa domain, the RTPase activity requuires an intact Walker B motif in the helicase core | Dengue virus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.6.1.15 | NS3 | - |
Dengue virus |
| 3.6.1.15 | nucleotide 5'-triphosphatase | - |
Dengue virus |
| 3.6.1.15 | protein NS3 | - |
Dengue virus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.15 | 25 | - |
helicase assay at | Dengue virus |
| 3.6.1.15 | 30 | - |
ATPase assay at | Dengue virus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.15 | 7.4 | - |
ATPase assay at | Dengue virus |
| 3.6.1.15 | 7.5 | - |
helicase assay at | Dengue virus |
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