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Literature summary extracted from

  • Pedrini, P.; Andreotti, E.; Guerrini, A.; Dean, M.; Fantin, G.; Giovannini, P.P.
    Xanthomonas maltophilia CBS 897.97 as a source of new 7beta- and 7alpha-hydroxysteroid dehydrogenases and cholylglycine hydrolase: improved biotransformations of bile acids (2006), Steroids, 71, 189-198.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.159 synthesis the enzyme is useful as biocatalyst in the reduction of 7-keto bile acids, co-operation with cholylglycine hydrolase, EC 3.5.1.24, overview Stenotrophomonas maltophilia

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.24 cholate uncompetitive inhibition of glycocholic acid hydrolysis Stenotrophomonas maltophilia
3.5.1.24 taurine uncompetitive inhibition of glycocholic acid hydrolysis Stenotrophomonas maltophilia
3.5.1.24 taurocholic acid competitive inhibition of glycocholic acid hydrolysis Stenotrophomonas maltophilia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.159 0.15
-
 NAD+ pH 7.5, 22°C Stenotrophomonas maltophilia
1.1.1.159 1
-
 cholic acid pH 7.5, 22°C Stenotrophomonas maltophilia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.159 additional information Stenotrophomonas maltophilia substrate specificity and cycles in bile acid pathways, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.159 Stenotrophomonas maltophilia
-
-
-
1.1.1.159 Stenotrophomonas maltophilia CBS 897.97
-
-
-
3.5.1.24 Bos taurus
-
-
-
3.5.1.24 Stenotrophomonas maltophilia
-
 i.e. Stenotrophomonas maltophilia
-
3.5.1.24 Stenotrophomonas maltophilia CBS 897.97
-
 i.e. Stenotrophomonas maltophilia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.159 native enzyme 175fold by anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction and affinity chromatography Stenotrophomonas maltophilia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.24 bile
-
 Bos taurus
-
3.5.1.24 commercial preparation from bile Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.159 additional information
-
-
 Stenotrophomonas maltophilia
1.1.1.159 70
-
 purified native enzyme Stenotrophomonas maltophilia

Storage Stability

EC Number Storage Stability Organism
1.1.1.159 4°C, purified enzyme, 50 mM sodium phosphate, pH 7.5, stable for 2 days Stenotrophomonas maltophilia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.159 (3alpha,5beta)-3-hydroxy-7,12-dioxocholan-24-oic acid + NADH
-
 Stenotrophomonas maltophilia (3alpha,5beta,7alpha)-3,7-dihydroxy-12-oxocholan-24-oic acid + NAD+
-
 r
1.1.1.159 3,7-diketocholic acid + NADH high activity Stenotrophomonas maltophilia 3-ketocholic acid + NAD+
-
 r
1.1.1.159 6-fluoro-7-ketohyocholic acid + NADH
-
 Stenotrophomonas maltophilia 6-fluoro-3alpha,6alpha,7alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+ + NAD+
-
 r
1.1.1.159 7-ketochenodeoxycholic acid + NADH
-
 Stenotrophomonas maltophilia 3alpha,7alpha-dihydroxy-5beta-cholanoic acid + NAD+
-
 r
1.1.1.159 7-ketohyocholic acid + NADH low activity Stenotrophomonas maltophilia 3alpha,6alpha,7alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
 r
1.1.1.159 cholic acid + NADH low activity in the reverse reaction Stenotrophomonas maltophilia 7-ketocholic acid + NAD+
-
 r
1.1.1.159 additional information substrate specificity and cycles in bile acid pathways, overview Stenotrophomonas maltophilia ?
-
 ?
3.5.1.24 glycochenodeoxycholate + H2O
-
 Bos taurus glycine + chenodeoxycholate
-
 ?
3.5.1.24 glycochenodeoxycholate + H2O
-
 Stenotrophomonas maltophilia glycine + chenodeoxycholate
-
 ?
3.5.1.24 glycochenodeoxycholate + H2O
-
 Stenotrophomonas maltophilia CBS 897.97 glycine + chenodeoxycholate
-
 ?
3.5.1.24 glycocholate + H2O
-
 Bos taurus glycine + cholate
-
 ?
3.5.1.24 glycocholate + H2O
-
 Stenotrophomonas maltophilia glycine + cholate
-
 ?
3.5.1.24 glycocholate + H2O
-
 Stenotrophomonas maltophilia CBS 897.97 glycine + cholate
-
 ?
3.5.1.24 glycodeoxycholate + H2O
-
 Bos taurus glycine + deoxycholate
-
 ?
3.5.1.24 glycodeoxycholate + H2O
-
 Stenotrophomonas maltophilia glycine + deoxycholate
-
 ?
3.5.1.24 glycodeoxycholate + H2O
-
 Stenotrophomonas maltophilia CBS 897.97 glycine + deoxycholate
-
 ?
3.5.1.24 glycohyodeoxycholate + H2O
-
 Stenotrophomonas maltophilia glycine + hyodeoxycholate
-
 ?
3.5.1.24 glycohyodeoxycholate + H2O
-
 Stenotrophomonas maltophilia CBS 897.97 glycine + hyodeoxycholate
-
 ?
3.5.1.24 glycoursodeoxycholate + H2O
-
 Stenotrophomonas maltophilia glycine + ursodeoxycholate
-
 ?
3.5.1.24 glycoursodeoxycholate + H2O
-
 Stenotrophomonas maltophilia CBS 897.97 glycine + ursodeoxycholate
-
 ?
3.5.1.24 taurochenodeoxycholate + H2O
-
 Bos taurus taurine + chenodeoxycholate
-
 ?
3.5.1.24 taurocholate + H2O
-
 Bos taurus taurine + cholate
-
 ?
3.5.1.24 taurodeoxycholate + H2O
-
 Bos taurus taurine + deoxycholate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.159 7alpha-HSDH
-
 Stenotrophomonas maltophilia
3.5.1.24 CGH
-
 Bos taurus
3.5.1.24 CGH
-
 Stenotrophomonas maltophilia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.159 28
-
 substrate cholic acid Stenotrophomonas maltophilia
3.5.1.24 50
-
-
 Stenotrophomonas maltophilia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.24 55
-
 75 min, completely stable Stenotrophomonas maltophilia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.159 7.5
-
-
 Stenotrophomonas maltophilia
3.5.1.24 8
-
-
 Stenotrophomonas maltophilia

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.159 NAD+
-
 Stenotrophomonas maltophilia
1.1.1.159 NADH
-
 Stenotrophomonas maltophilia

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.24 2.5
-
 taurine pH 8.0, 50°C Stenotrophomonas maltophilia
3.5.1.24 2.5
-
 taurocholic acid pH 8.0, 50°C Stenotrophomonas maltophilia
3.5.1.24 4.7
-
 cholate pH 8.0, 50°C Stenotrophomonas maltophilia