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Literature summary extracted from

  • Parent, M.; Madore, E.; MacLaren, L.A.; Fortier, M.A.
    15-Hydroxyprostaglandin dehydrogenase in the bovine endometrium during the oestrous cycle and early pregnancy (2006), Reproduction, 131, 573-582.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.141 15-PGDH, DNA and amino acid sequence determination and analysis, functional overexpression of His-tagged lung enzyme in Escherichia coli Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.141 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ Bos taurus
-
 (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.141 Bos taurus Q309F3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.141 recombinant His-tagged lung enzyme from Escherichia coli by nickel affinity chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.141 glandular epithelium
-
 Bos taurus
-
1.1.1.141 lung
-
 Bos taurus
-
1.1.1.141 uterine endometrium the enzyme is expressed mainly in the glandular epithelium, enzyme activity during the oestrous cycle and early pregnancy, overview Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.141 additional information
-
 enzyme activity in the endometrium during the oestrous cycle and early pregnancy, overview Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.141 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+
-
 Bos taurus (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?
1.1.1.141 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ i.e. 15-hydroxyprostaglandin or (15S)-PGE2 Bos taurus (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.141 15-hydroxyprostaglandin dehydrogenase
-
 Bos taurus
1.1.1.141 15-PGDH
-
 Bos taurus
1.1.1.141 NAD+ dependent 15-hydroxyprostaglandin dehydrogenase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.141 25
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.141 9
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.141 NAD+
-
 Bos taurus