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Literature summary extracted from

  • Porcelli, M.; Moretti, M.A.; Concilio, L.; Forte, S.; Merlino, A.; Graziano, G.; Cacciapuoti, G.
    S-Adenosylhomocysteine hydrolase from the archaeon Pyrococcus furiosus: biochemical characterization and analysis of protein structure by comparative molecular modeling (2005), Proteins, 58, 815-825.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.13.2.1 expression in Escherichia coli Pyrococcus furiosus
3.13.2.1 expression of mutant enzymes T60C, T60S, T60A and Q85A in Escherichia coli Homo sapiens
3.13.2.1 expression of mutant enzymesC59T, C59S and A84Q in Escherichia coli Pyrococcus furiosus

Protein Variants

EC Number Protein Variants Comment Organism
3.13.2.1 A84Q kcat/Km is 1.2fold higher then wild-type value. Ki-value for noraristeromycin is 1.2fold lower than wild-type value, Ki-value for 2-fluoronoraristeromycin is 1.3fold lower than wild-type value Pyrococcus furiosus
3.13.2.1 C59S kcat/Km is 1.5fold lower then wild-type value. Ki-value for noraristeromycin is 1.1fold higher than wild-type value, Ki-value for 2-fluoronoraristeromycin is 1.2fold lower than wild-type value Pyrococcus furiosus
3.13.2.1 C59T kcat/Km is 1.1fold higher then wild-type value. Ki-value for noraristeromycin is 1.4fold lower than wild-type value, Ki-value for 2-fluoronoraristeromycin more than 6.5fold higher than wild-type value Pyrococcus furiosus
3.13.2.1 Q85A kcat/Km is 13.45fold lower then wild-type value. Ki-value for noraristeromycin is 4.46fold higher than wild-type value, Ki-value for 2-fluoronoraristeromycin is 5.6fold higher than wild-type value Homo sapiens
3.13.2.1 T60A kcat/Km is 1.23 fold lower then wild-type value. Ki-value for noraristeromycin is 3.3fold lower than wild-type value, Ki-value for 2-fluoronoraristeromycin is 23fold lower than wild-type value Homo sapiens
3.13.2.1 T60C kcat/Km is 2.3fold lower then wild-type value. Ki-value for noraristeromycin is 1.2fold higher than wild-type value, Ki-value for 2-fluoronoraristeromycin 11fold lower than wild-type value Homo sapiens
3.13.2.1 T60S kcat/Km is 1.6fold higher then wild-type value. Ki-value for noraristeromycin is 2.3fold lower than wild-type value, Ki-value for 2-fluoronoraristeromycin is 50fold lower than wild-type value Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.13.2.1 2-fluoronoraristeromycin
-
 Homo sapiens
3.13.2.1 2-fluoronoraristeromycin
-
 Pyrococcus furiosus
3.13.2.1 dithiothreitol 0.8 M, reduces activity Pyrococcus furiosus
3.13.2.1 noraristeromycin
-
 Homo sapiens
3.13.2.1 noraristeromycin
-
 Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.2.1 0.00024
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme A84Q Pyrococcus furiosus
3.13.2.1 0.00025
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme C59T Pyrococcus furiosus
3.13.2.1 0.00029
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, wild-type enzyme Pyrococcus furiosus
3.13.2.1 0.00039
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme C59S Pyrococcus furiosus
3.13.2.1 0.0007
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme T60S Homo sapiens
3.13.2.1 0.00129
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme T60A Homo sapiens
3.13.2.1 0.0014
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, wild-type enzyme Homo sapiens
3.13.2.1 0.00172
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme T60C Homo sapiens
3.13.2.1 0.0048
-
 adenosine 80°C, pH 7.4 Pyrococcus furiosus
3.13.2.1 0.00555
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme Q85A Homo sapiens
3.13.2.1 0.014
-
 S-adenosyl-DL-homocysteine 80°C, pH 7.4 Pyrococcus furiosus
3.13.2.1 0.14
-
 DL-homocysteine 80°C, pH 7.4 Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.13.2.1 47000
-
 4 * 47000, SDS-PAGE Pyrococcus furiosus
3.13.2.1 190000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.2.1 S-adenosyl-L-homocysteine + H2O Pyrococcus furiosus the enzyme catalyzes the breakdown of S-adenosylhomocysteine, a powerful inhibitor of most transmethylation reactions, to adenosine and L-homocysteine L-homocysteine + adenosine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
3.13.2.1 Homo sapiens
-
-
-
3.13.2.1 Pyrococcus furiosus
-
-
-
3.13.2.1 Pyrococcus furiosus P50251
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.2.1
-
 Homo sapiens
3.13.2.1
-
 Pyrococcus furiosus
3.13.2.1 recombinant Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.13.2.1 0.033
-
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.2.1 DL-homocysteine + adenosine
-
 Pyrococcus furiosus S-adenosyl-DL-homocysteine
-
 ?
3.13.2.1 S-adenosyl-DL-homocysteine + H2O
-
 Homo sapiens DL-homocysteine + adenosine
-
 ?
3.13.2.1 S-adenosyl-DL-homocysteine + H2O
-
 Pyrococcus furiosus DL-homocysteine + adenosine
-
 ?
3.13.2.1 S-adenosyl-L-homocysteine + H2O
-
 Pyrococcus furiosus L-homocysteine + adenosine
-
 r
3.13.2.1 S-adenosyl-L-homocysteine + H2O the enzyme catalyzes the breakdown of S-adenosylhomocysteine, a powerful inhibitor of most transmethylation reactions, to adenosine and L-homocysteine Pyrococcus furiosus L-homocysteine + adenosine
-
 r

Subunits

EC Number Subunits Comment Organism
3.13.2.1 homotetramer 4 * 47000, SDS-PAGE Pyrococcus furiosus
3.13.2.1 tetramer 4 * 47000, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.13.2.1 AdoHcy hydrolase
-
 Pyrococcus furiosus
3.13.2.1 AdoHcyHD
-
 Pyrococcus furiosus
3.13.2.1 HsSAHH
-
 Homo sapiens
3.13.2.1 PF0343
-
 Pyrococcus furiosus
3.13.2.1 PfAdoHcyHD
-
 Pyrococcus furiosus
3.13.2.1 PfSAHH
-
 Pyrococcus furiosus
3.13.2.1 S-adenosylhomocysteine hydrolase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.13.2.1 80
-
 assay at Pyrococcus furiosus
3.13.2.1 95
-
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.13.2.1 75 105 75°C: about 50% of maximal activity, 105°C: about 45% of maximal activity Pyrococcus furiosus
3.13.2.1 75 105 75°C: about 50% of maximalm activity, 105°C: about 45% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.13.2.1 90
-
 1 h, stable Pyrococcus furiosus
3.13.2.1 90
-
 1 h, no loss of activity Pyrococcus furiosus
3.13.2.1 95
-
 half-life: 50 min Pyrococcus furiosus
3.13.2.1 98
-
 melting temperature Pyrococcus furiosus
3.13.2.1 100
-
 half-life: 5 min Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.13.2.1 0.053
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme C59S Pyrococcus furiosus
3.13.2.1 0.054
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme C59T Pyrococcus furiosus
3.13.2.1 0.057
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme A84Q Pyrococcus furiosus
3.13.2.1 0.058
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, wild-type enzyme Pyrococcus furiosus
3.13.2.1 1
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme Q85A Homo sapiens
3.13.2.1 1.87
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme T60C Homo sapiens
3.13.2.1 2.55
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme T60A Homo sapiens
3.13.2.1 2.8
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme T60S Homo sapiens
3.13.2.1 3.45
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, wild-type enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.13.2.1 7.4
-
-
 Pyrococcus furiosus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.13.2.1 0.000024
-
 noraristeromycin 30°C, pH 7.6, mutant enzyme C59T Pyrococcus furiosus
3.13.2.1 0.000028
-
 noraristeromycin 30°C, pH 7.6, mutant enzyme T60A Homo sapiens
3.13.2.1 0.000029
-
 noraristeromycin 30°C, pH 7.6, mutant enzyme A84Q Pyrococcus furiosus
3.13.2.1 0.000034
-
 noraristeromycin 30°C, pH 7.6, wild-type enzyme Pyrococcus furiosus
3.13.2.1 0.000036
-
 noraristeromycin 30°C, pH 7.6, mutant enzyme C59S Pyrococcus furiosus
3.13.2.1 0.000039
-
 noraristeromycin 30°C, pH 7.6, mutant enzyme T60S Homo sapiens
3.13.2.1 0.00006
-
 2-fluoronoraristeromycin 30°C, pH 7.6, mutant enzyme T60S Homo sapiens
3.13.2.1 0.000092
-
 noraristeromycin 30°C, pH 7.6, wild-type enzyme Homo sapiens
3.13.2.1 0.0001
-
 noraristeromycin 30°C, pH 7.6, mutant enzyme T60C Homo sapiens
3.13.2.1 0.000116
-
 2-fluoronoraristeromycin 30°C, pH 7.6, mutant enzyme A84Q Pyrococcus furiosus
3.13.2.1 0.000129
-
 2-fluoronoraristeromycin 30°C, pH 7.6, mutant enzyme T60A Homo sapiens
3.13.2.1 0.00013
-
 2-fluoronoraristeromycin 30°C, pH 7.6, mutant enzyme C59S Pyrococcus furiosus
3.13.2.1 0.000154
-
 2-fluoronoraristeromycin 30°C, pH 7.6, wild-type enzyme Pyrococcus furiosus
3.13.2.1 0.000272
-
 2-fluoronoraristeromycin 30°C, pH 7.6, mutant enzyme T60C Homo sapiens
3.13.2.1 0.00041
-
 noraristeromycin 30°C, pH 7.6, mutant enzyme Q85A Homo sapiens
3.13.2.1 0.003
-
 2-fluoronoraristeromycin 30°C, pH 7.6, wild-type enzyme Homo sapiens
3.13.2.1 0.0168
-
 2-fluoronoraristeromycin 30°C, pH 7.6, mutant enzyme Q85A Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.13.2.1 Pyrococcus furiosus isoelectric focusing, pH-range 3-10 and 4.0-6.5
-
 5.1
3.13.2.1 Pyrococcus furiosus isoelectric focusing, pH ranges 3–10 and 4.0–6.5
-
 5.1

General Information

EC Number General Information Comment Organism
3.13.2.1 physiological function the enzyme catalyzes the breakdown of S-adenosylhomocysteine, a powerful inhibitor of most transmethylation reactions, to adenosine and L-homocysteine Pyrococcus furiosus