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Literature summary extracted from

  • Liggieri, C.; Arribere, M.C.; Trejo, S.A.; Canals, F.; Aviles, F.X.; Priolo, N.S.
    Purification and biochemical characterization of asclepain c I from the latex of Asclepias curassavica L (2004), Protein J., 23, 403-411.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.7 E-64
-
 Asclepias curassavica
3.4.22.7 NaCl 1 M, 70% loss of activity Asclepias curassavica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.7 0.73
-
 L-pyroglutamyl-L-phenylalanyl-L-leucine-4-nitroanilide pH 8.5, 42°C, Lineweaver-Burk equation Asclepias curassavica
3.4.22.7 0.82
-
 L-pyroglutamyl-L-phenylalanyl-L-leucine-4-nitroanilide pH 8.5, 42°C, Eddie-Hofstee equation Asclepias curassavica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.7 23200
-
 x * 23200, mass spectrometry Asclepias curassavica

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.7 Asclepias curassavica
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.7 latex
-
 Asclepias curassavica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.7 10880
-
 pH 8.5, 42°C Asclepias curassavica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.7 azocasein + H2O
-
 Asclepias curassavica ?
-
 ?
3.4.22.7 L-pyroglutamyl-L-phenylalanyl-L-leucine-4-nitroanilide + H2O
-
 Asclepias curassavica ?
-
 ?
3.4.22.7 N-carbobenzoxy-L-Ala 4-nitrophenyl ester + H2O 47% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester Asclepias curassavica N-carbobenzoxy-L-Ala + 4-nitrophenol
-
 ?
3.4.22.7 N-carbobenzoxy-L-Gln 4-nitrophenyl ester + H2O
-
 Asclepias curassavica N-carbobenzoxy-L-Gln + 4-nitrophenol
-
 ?
3.4.22.7 N-carbobenzoxy-L-Ile 4-nitrophenyl ester + H2O 59% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester Asclepias curassavica N-carbobenzoxy-L-Ile + 4-nitrophenol
-
 ?
3.4.22.7 N-carbobenzoxy-L-Lys 4-nitrophenyl ester + H2O 78% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester Asclepias curassavica N-carbobenzoxy-L-Lys + 4-nitrophenol
-
 ?
3.4.22.7 N-carbobenzoxy-L-Tyr 4-nitrophenyl ester + H2O 51% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester Asclepias curassavica N-carbobenzoxy-L-Tyr + 4-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.7 ? x * 23200, mass spectrometry Asclepias curassavica

Synonyms

EC Number Synonyms Comment Organism
3.4.22.7 asclepain cI
-
 Asclepias curassavica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.7 37
-
 2 h at pH 8.5, stable, 4 h, 30% loss of activity Asclepias curassavica
3.4.22.7 40
-
 2 h, stable Asclepias curassavica
3.4.22.7 50
-
 2 h, stable Asclepias curassavica
3.4.22.7 60
-
 2 h, stable Asclepias curassavica
3.4.22.7 70
-
 40 min, complete inactivation Asclepias curassavica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.7 8.5
-
-
 Asclepias curassavica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.7 7.5 9
-
 Asclepias curassavica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.7 8.5
-
 2 h at 37°C, stable, 4 h, 30% loss of activity Asclepias curassavica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.22.7 Asclepias curassavica higher than, isoelectric focusing
-
 9.3