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Literature summary extracted from

  • Kim, D.E.; Kim, K.H.; Bae, Y.J.; Lee, J.H.; Jang, Y.H.; Nam, S.W.
    Purification and characterization of the recombinant arylsulfatase cloned from Pseudoalteromonas carrageenovora (2005), Protein Expr. Purif., 39, 107-115.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.6.1 overexpression in Escherichia coli BL21 Pseudoalteromonas carrageenovora

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.1 EDTA 0.1 mM EDTA 46.8% relative activity, 0.5 mM EDTA 39.6% relative activity and 1.0 mM EDTA 30.5% relative activity compared to the control Pseudoalteromonas carrageenovora
3.1.6.1 PMSF 0.1 mM PMSF 92.7% relative activity, 0.5 mM PMSF 82.6% relative activity and 1.0 mM PMSF 73.5% relative activity compared to the control Pseudoalteromonas carrageenovora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.1 0.67
-
 p-Nitrophenyl sulfate 6.5 nM purified arylsulfatase incubated at 45°C and at pH 7.0 in the presence of 10 mM sulfate Pseudoalteromonas carrageenovora
3.1.6.1 1.15
-
 p-Nitrophenyl sulfate 6.5 nM purified arylsulfatase incubated at 45°C and at pH 7.0 Pseudoalteromonas carrageenovora

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.1 33000
-
 SDS-PAGE Pseudoalteromonas carrageenovora

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.1 Pseudoalteromonas carrageenovora
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.6.1 DEAE-cellulose anion-exchange and Heparin-Sepharose CL 6B affinity chromatography, all steps at 4°C Pseudoalteromonas carrageenovora

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.6.1 468
-
 7.1 mM p-nitrophenyl sulfate at 45°C and at pH 8.5, after Heparin-Sepharose column chromatography Pseudoalteromonas carrageenovora

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.1 p-nitrophenyl sulfate + H2O
-
 Pseudoalteromonas carrageenovora p-nitrophenol + sulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.6.1 arylsulfatase
-
 Pseudoalteromonas carrageenovora

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.6.1 45
-
 purified arylsulfatase analysed at pH 7.0 and temperature between 10 to 80°C, p-nitrophenyl sulfate as substrate Pseudoalteromonas carrageenovora

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.6.1 1000
-
 p-Nitrophenyl sulfate 6.5 nM purified arylsulfatase, at 45°C and pH 7.0 Pseudoalteromonas carrageenovora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.1 7
-
 activity of purified arylsulfatase analysed at 40°C and at pH 4.5 to 10, p-nitrophenyl sulfate as substrate Pseudoalteromonas carrageenovora