Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Masuo, N.; Ito, K.; Yoshimune, K.; Hoshino, M.; Matsushima, K.; Koyama, Y.; Moriguchi, M.
    Molecular cloning, overexpression, and purification of Micrococcus luteus K-3-type glutaminase from Aspergillus oryzae RIB40 (2004), Protein Expr. Purif., 38, 272-278.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.2 gene AoglsA, from strain RIB40, DNA and amino acid sequence determination and analysis, functional overexpression in Saccharomyces cerevisiae cell wall and in Escherichia coli as soluble protein Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.2 49883
-
 x * 49883, amino acid sequence calculation Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.2 L-glutamine + H2O Aspergillus oryzae
-
 L-glutamate + NH3
-
 ?
3.5.1.2 L-glutamine + H2O Aspergillus oryzae RIB 40
-
 L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.2 Aspergillus oryzae Q66V97
-
-
3.5.1.2 Aspergillus oryzae RIB 40 Q66V97
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.2 recombinant enzyme 5.9fold from Escherichia coli by anion exchange, glutamine affinity, and hydrophobic interaction chromatography to homogeneity Aspergillus oryzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.2 additional information
-
 activity of different enzyme constructs recombinantly expressed in different Escherichia coli strains, overview Aspergillus oryzae
3.5.1.2 11.1
-
 recombinant enzyme in Escherichia coli strain JM109 Aspergillus oryzae
3.5.1.2 730
-
 purified recombinant enzyme Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.2 L-glutamine + H2O
-
 Aspergillus oryzae L-glutamate + NH3
-
 ?
3.5.1.2 L-glutamine + H2O
-
 Aspergillus oryzae RIB 40 L-glutamate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.2 ? x * 49883, amino acid sequence calculation Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.5.1.2 AoGls
-
 Aspergillus oryzae
3.5.1.2 Micrococcus luteus K-3-type glutaminase
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.2 30
-
 assay at Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.2 7.5
-
 assay at Aspergillus oryzae