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Literature summary extracted from

  • Shrestha, B.; Blondeau, K.; Stevens, W.F.; Hegarat, F.L.
    Expression of chitin deacetylase from Colletotrichum lindemuthianum in Pichia pastoris: purification and characterization (2004), Protein Expr. Purif., 38, 196-204.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.41 synthesis the enzyme might be useful for large scale production of chitosan from chitin Colletotrichum lindemuthianum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.41 expression of His6-tagged enzyme in Pichia pastoris strain GS115, secretion of the recombinant enzyme to the culture supernatant Colletotrichum lindemuthianum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.41 Ca2+ inhibitory at higher concentration Colletotrichum lindemuthianum
3.5.1.41 Cu2+ highly inhibitory at higher concentration Colletotrichum lindemuthianum
3.5.1.41 Fe2+ highly inhibitory at higher concentration Colletotrichum lindemuthianum
3.5.1.41 Mg2+ inhibitory at higher concentration Colletotrichum lindemuthianum
3.5.1.41 Ni2+ potent inhibition Colletotrichum lindemuthianum
3.5.1.41 Zn2+ potent inhibition Colletotrichum lindemuthianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.41 1.5
-
 hexa-N-acetyl-chitohexaose pH 8.0, 50°C, recombinant enzyme Colletotrichum lindemuthianum
3.5.1.41 5.7
-
 penta-N-acetyl-chitopentaose pH 8.0, 50°C, recombinant enzyme Colletotrichum lindemuthianum
3.5.1.41 8.9
-
 tetra-N-acetyl-chitotetraose pH 8.0, 50°C, recombinant enzyme Colletotrichum lindemuthianum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.41 Co2+ activates by 26% Colletotrichum lindemuthianum
3.5.1.41 additional information Li+ and Mn2+ have no effect on enzyme activity Colletotrichum lindemuthianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.41 25200
-
 x * 25200, recombinant His6-tagged enzyme, SDS-PAGE Colletotrichum lindemuthianum

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.41 Colletotrichum lindemuthianum Q6DWK3
-
-
3.5.1.41 Colletotrichum lindemuthianum UPS9 Q6DWK3
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.41 no glycoprotein recombinant His6-tagged enzyme Colletotrichum lindemuthianum
3.5.1.41 proteolytic modification the enzyme contains a signal sequence which is cleaved at Gln28 Colletotrichum lindemuthianum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.41 recombinant His6-tagged enzyme 364fold from Pichia pastoris strain GS115 culture supernatant by the single step process of nickel affinity chromatography Colletotrichum lindemuthianum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.41 additional information
-
 substrate specificity Colletotrichum lindemuthianum
3.5.1.41 0.2
-
 recombinant His6-tagged enzyme in Pichia pastoris cell culture supernatant Colletotrichum lindemuthianum
3.5.1.41 135.6
-
 purified recombinant His6-tagged enzyme, substrate penta-N-acetyl-chitopentaose Colletotrichum lindemuthianum
3.5.1.41 138.5
-
 purified recombinant His6-tagged enzyme, substrate tetra-N-acetyl-chitotetraose Colletotrichum lindemuthianum
3.5.1.41 184.4
-
 purified recombinant His6-tagged enzyme, substrate hexa-N-acetyl-chitohexaose Colletotrichum lindemuthianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.41 hexa-N-acetyl-chitohexaose + H2O
-
 Colletotrichum lindemuthianum chitohexaose + acetate
-
 ?
3.5.1.41 hexa-N-acetyl-chitohexaose + H2O
-
 Colletotrichum lindemuthianum UPS9 chitohexaose + acetate
-
 ?
3.5.1.41 additional information substrate specificity, overview, no activity with chitotriose Colletotrichum lindemuthianum ?
-
 ?
3.5.1.41 additional information substrate specificity, overview, no activity with chitotriose Colletotrichum lindemuthianum UPS9 ?
-
 ?
3.5.1.41 penta-N-acetyl-chitopentaose + H2O
-
 Colletotrichum lindemuthianum chitopentaose + acetate
-
 ?
3.5.1.41 penta-N-acetyl-chitopentaose + H2O
-
 Colletotrichum lindemuthianum UPS9 chitopentaose + acetate
-
 ?
3.5.1.41 tetra-N-acetyl-chitotetraose + H2O
-
 Colletotrichum lindemuthianum chitotetraose + acetate
-
 ?
3.5.1.41 tetra-N-acetyl-chitotetraose + H2O
-
 Colletotrichum lindemuthianum UPS9 chitotetraose + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.41 ? x * 25200, recombinant His6-tagged enzyme, SDS-PAGE Colletotrichum lindemuthianum
3.5.1.41 More comparison of enzyme amino acid sequences of strain UPS9 with those of strain ATCC 56676, overview Colletotrichum lindemuthianum

Synonyms

EC Number Synonyms Comment Organism
3.5.1.41 CDA
-
 Colletotrichum lindemuthianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.41 60
-
 with substrate tetra-N-acetyl-chitotetraose Colletotrichum lindemuthianum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.41 40 75 half-maximal activity at 40°C and 75°C, 30% of maximal activity at 80°C Colletotrichum lindemuthianum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.41 80
-
 half-life: 1 h Colletotrichum lindemuthianum
3.5.1.41 90
-
 half-life: 30 min Colletotrichum lindemuthianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.41 8
-
 with substrate tetra-N-acetyl-chitotetraose Colletotrichum lindemuthianum