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Literature summary extracted from

  • Crepin, V.F.; Faulds, C.B.; Connerton, I.F.
    Production and characterization of the Talaromyces stipitatus feruloyl esterase FAEC in Pichia pastoris: identification of the nucleophilic serine (2003), Protein Expr. Purif., 29, 176-184.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.73 nutrition broad range of application in the agri-food industries, production of ferulic as food preservative and as potential precursor for the production of flavor and photo-active agents Talaromyces stipitatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 expressed in Pichia pastoris Talaromyces stipitatus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.73 additional information additional amino acids at the N-terminus do not influence the activity Talaromyces stipitatus
3.1.1.73 S166A complete loss of activity Talaromyces stipitatus
3.1.1.73 S465A no effect on activity Talaromyces stipitatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.01
-
 methyl p-coumarate pH 6.0, 37°C Talaromyces stipitatus
3.1.1.73 0.04
-
 methyl ferulate pH 6.0, 37°C Talaromyces stipitatus
3.1.1.73 0.37
-
 methyl sinapinate pH 6.0, 37°C Talaromyces stipitatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular
-
 Talaromyces stipitatus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 55340
-
 1 * 55340, calculated from the deduced amino acid sequence, 1 * 72000, SDS-PAGE Talaromyces stipitatus
3.1.1.73 72000
-
 1 * 55340, calculated from the deduced amino acid sequence, 1 * 72000, SDS-PAGE Talaromyces stipitatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 feruloyl polysaccharide + H2O Talaromyces stipitatus involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?
3.1.1.73 feruloyl polysaccharide + H2O Talaromyces stipitatus CBS 375.48 involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?
3.1.1.73 hemicellulose + H2O Talaromyces stipitatus involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + lignin + ?
-
 ?
3.1.1.73 hemicellulose + H2O Talaromyces stipitatus CBS 375.48 involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + lignin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Talaromyces stipitatus Q70Y21
-
-
3.1.1.73 Talaromyces stipitatus CBS 375.48 Q70Y21
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.73 glycoprotein
-
 Talaromyces stipitatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Talaromyces stipitatus ferulic acid + ?
-
 ?
3.1.1.73 feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Talaromyces stipitatus CBS 375.48 ferulic acid + ?
-
 ?
3.1.1.73 hemicellulose + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Talaromyces stipitatus ferulic acid + lignin + ?
-
 ?
3.1.1.73 hemicellulose + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Talaromyces stipitatus CBS 375.48 ferulic acid + lignin + ?
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Talaromyces stipitatus caffeic acid + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Talaromyces stipitatus CBS 375.48 caffeic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Talaromyces stipitatus ferulic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Talaromyces stipitatus CBS 375.48 ferulic acid + methanol
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Talaromyces stipitatus p-coumaric acid + methanol
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Talaromyces stipitatus sinapic acid + methanol
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Talaromyces stipitatus CBS 375.48 sinapic acid + methanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 monomer 1 * 55340, calculated from the deduced amino acid sequence, 1 * 72000, SDS-PAGE Talaromyces stipitatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 0.04 1.97 methyl sinapinate pH 6.0, 37°C Talaromyces stipitatus
3.1.1.73 9.26
-
 methyl sinapinate pH 6.0, 37°C Talaromyces stipitatus
3.1.1.73 9.55
-
 methyl p-coumarate pH 6.0, 37°C Talaromyces stipitatus
3.1.1.73 9.65
-
 methyl ferulate pH 6.0, 37°C Talaromyces stipitatus