BRENDA - Enzyme Database show

Isoenzymatic forms of human cytidine deaminase

Vincenzetti, S.; Mariani, P.L.; Cammertoni, N.; Polzonetti, V.; Natalini, P.; Quadrini, B.; Volpini, R.; Vita, A.; Protein Eng. Des. Sel. 17, 871-877 (2004)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
(cytidyl)acryloylaminohexanoic acid
i.e. CV6
Homo sapiens
3.5.4.5
3,4,5,6-Tetrahydrouridine
-
Homo sapiens
3.5.4.5
5,6-Dihydrouridine
-
Homo sapiens
3.5.4.5
5-fluorozebularine
-
Homo sapiens
3.5.4.5
cytidine monophosphate
-
Homo sapiens
3.5.4.5
uridine
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.03
-
2'-deoxycytidine
native enzyme, 37°C
Homo sapiens
3.5.4.5
0.04
-
2'-deoxycytidine
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.04
-
cytidine
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
3.5.4.5
0.042
-
2'-deoxycytidine
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
3.5.4.5
0.042
-
cytidine
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.087
-
5-iododeoxycytidine
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
3.5.4.5
0.1
-
5-iododeoxycytidine
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.11
-
cytosine arabinoside
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.15
-
cytosine arabinoside
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
3.5.4.5
0.28
-
5-azacytidine
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.31
-
5-azacytidine
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
15000
-
4 * 15000, SDS-PAGE
Homo sapiens
3.5.4.5
60000
-
gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.5
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.5
placenta
different arrangement of subunit isoforms CDA1 and CDA2 results in five isoforms differing only in isoelectric point, native and recombinant enzyme
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
2'-deoxycytidine + H2O
-
670747
Homo sapiens
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
670747
Homo sapiens
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-iododeoxycytidine + H2O
-
670747
Homo sapiens
5-iododeoxyuridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
670747
Homo sapiens
uridine + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
670747
Homo sapiens
uridine arabinoside + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.5
tetramer
4 * 15000, SDS-PAGE
Homo sapiens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.4.5
0.00006
-
5-fluorozebularine
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
3.5.4.5
0.0003
-
5-fluorozebularine
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.007
-
3,4,5,6-Tetrahydrouridine
tetramer of recombinant subunit CDA1, 37°C; tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.015
-
5,6-Dihydrouridine
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
3.5.4.5
0.034
-
(cytidyl)acryloylaminohexanoic acid
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
3.5.4.5
0.04
-
5,6-Dihydrouridine
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.045
-
(cytidyl)acryloylaminohexanoic acid
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.3
-
cytidine monophosphate
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.31
-
uridine
tetramer of recombinant subunit CDA1, 37°C; tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.39
-
cytidine monophosphate
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.5.4.5
Homo sapiens
tetramer of recombinant subunit CDA2, isoelectric focusing
-
4.5
3.5.4.5
Homo sapiens
5 different isoforms of native enzyme, isoelectric focusing
4.9
4.55
3.5.4.5
Homo sapiens
tetramer of recombinant subunit CDA1, isoelectric focusing
-
5
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
(cytidyl)acryloylaminohexanoic acid
i.e. CV6
Homo sapiens
3.5.4.5
3,4,5,6-Tetrahydrouridine
-
Homo sapiens
3.5.4.5
5,6-Dihydrouridine
-
Homo sapiens
3.5.4.5
5-fluorozebularine
-
Homo sapiens
3.5.4.5
cytidine monophosphate
-
Homo sapiens
3.5.4.5
uridine
-
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.4.5
0.00006
-
5-fluorozebularine
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
3.5.4.5
0.0003
-
5-fluorozebularine
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.007
-
3,4,5,6-Tetrahydrouridine
tetramer of recombinant subunit CDA1, 37°C; tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.015
-
5,6-Dihydrouridine
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
3.5.4.5
0.034
-
(cytidyl)acryloylaminohexanoic acid
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
3.5.4.5
0.04
-
5,6-Dihydrouridine
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.045
-
(cytidyl)acryloylaminohexanoic acid
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.3
-
cytidine monophosphate
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.31
-
uridine
tetramer of recombinant subunit CDA1, 37°C; tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.39
-
cytidine monophosphate
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.03
-
2'-deoxycytidine
native enzyme, 37°C
Homo sapiens
3.5.4.5
0.04
-
2'-deoxycytidine
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.04
-
cytidine
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
3.5.4.5
0.042
-
2'-deoxycytidine
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
3.5.4.5
0.042
-
cytidine
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.087
-
5-iododeoxycytidine
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
3.5.4.5
0.1
-
5-iododeoxycytidine
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.11
-
cytosine arabinoside
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.15
-
cytosine arabinoside
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
3.5.4.5
0.28
-
5-azacytidine
tetramer of recombinant subunit CDA2, 37°C
Homo sapiens
3.5.4.5
0.31
-
5-azacytidine
tetramer of recombinant subunit CDA1, 37°C
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
15000
-
4 * 15000, SDS-PAGE
Homo sapiens
3.5.4.5
60000
-
gel filtration
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.5
placenta
different arrangement of subunit isoforms CDA1 and CDA2 results in five isoforms differing only in isoelectric point, native and recombinant enzyme
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
2'-deoxycytidine + H2O
-
670747
Homo sapiens
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
670747
Homo sapiens
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-iododeoxycytidine + H2O
-
670747
Homo sapiens
5-iododeoxyuridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
670747
Homo sapiens
uridine + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
670747
Homo sapiens
uridine arabinoside + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.5
tetramer
4 * 15000, SDS-PAGE
Homo sapiens
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.5.4.5
Homo sapiens
tetramer of recombinant subunit CDA2, isoelectric focusing
-
4.5
3.5.4.5
Homo sapiens
5 different isoforms of native enzyme, isoelectric focusing
4.9
4.55
3.5.4.5
Homo sapiens
tetramer of recombinant subunit CDA1, isoelectric focusing
-
5