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Literature summary extracted from

  • Demidyuk, I.V.; Romanova, D.V.; Nosovskaya, E.A.; Chestukhina, G.G.; Kuranova, I.P.; Kostrov, S.V.
    Modification of substrate-binding site of glutamyl endopeptidase from Bacillus intermedius (2004), Protein Eng. Des. Sel., 17, 411-416.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.19 H186T no effect on enzyme specificity, 4.9fold increase in KM-value, 600fold decrease in kcat-value Bacillus intermedius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.19 0.67
-
 benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-L-Glu-4-nitroanilide wild-type, pH 8.5, 37°C Bacillus intermedius
3.4.21.19 3.3
-
 benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-L-Glu-4-nitroanilide mutant H186T, pH 8.5, 37°C Bacillus intermedius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.19 23000
-
 x * 23000, SDS-PAGE Bacillus intermedius

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.19 Bacillus intermedius
-
-
-

Storage Stability

EC Number Storage Stability Organism
3.4.21.19 4°C, 10 months, no loss of activity for mutant H186T Bacillus intermedius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.19 azocasein + H2O
-
 Bacillus intermedius ?
-
 ?
3.4.21.19 benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-L-Glu-4-nitroanilide + H2O
-
 Bacillus intermedius benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-L-Glu + 4-nitroaniline
-
 ?
3.4.21.19 additional information no cleavage of certain substrates of chymotrypsin Bacillus intermedius ?
-
 ?
3.4.21.19 Oxidized insulin B-chain + H2O
-
 Bacillus intermedius ? cleavage of bond E13-A14 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.19 ? x * 23000, SDS-PAGE Bacillus intermedius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.19 50
-
 mutant H186T, 150 min, no loss of activity Bacillus intermedius

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.19 0.027
-
 benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-L-Glu-4-nitroanilide mutant H186T, pH 8.5, 37°C Bacillus intermedius
3.4.21.19 16.6
-
 benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-L-Glu-4-nitroanilide wild-type, pH 8.5, 37°C Bacillus intermedius