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Literature summary extracted from

  • Skaar, E.P.; Tobiason, D.M.; Quick, J.; Judd, R.C.; Weissbach, H.; Etienne, F.; Brot, N.; Seifert, H.S.
    The outer membrane localization of the Neisseria gonorrhoeae MsrA/B is involved in survival against reactive oxygen species (2002), Proc. Natl. Acad. Sci. USA, 99, 10108-10113.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.12 gene pilB, transposon insertion, truncated PilB enzyme forms of the enzyme lacking the MsrA domain from strain MS11, variant VD300, overexpression of the His-tagged PilB forms in Escherichia coli strain Xl-1 blue Neisseria gonorrhoeae

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.12 additional information construction of truncated PilB domain forms, which show decreased survival of the organism to reactive oxygen species, the mutations do not affect piliation, pilin production, or adherence, overview Neisseria gonorrhoeae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.11 outer membrane
-
 Neisseria gonorrhoeae 19867
-
1.8.4.12 outer membrane
-
 Neisseria gonorrhoeae 19867
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.12 additional information Neisseria gonorrhoeae PilB affects the survival of the organism to reactive oxygen species, PilB is not involved in piliation, pilin production, or adherence ?
-
 ?
1.8.4.12 additional information Neisseria gonorrhoeae MS11 PilB affects the survival of the organism to reactive oxygen species, PilB is not involved in piliation, pilin production, or adherence ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.11 Neisseria gonorrhoeae
-
 variant VD300, bifunctional enzyme MsrA/B, gene pilA
-
1.8.4.11 Neisseria gonorrhoeae MS11
-
 variant VD300, bifunctional enzyme MsrA/B, gene pilA
-
1.8.4.12 Neisseria gonorrhoeae
-
 variant VD300, bifunctional enzyme MsrA/B, gene pilB
-
1.8.4.12 Neisseria gonorrhoeae MS11
-
 variant VD300, bifunctional enzyme MsrA/B, gene pilB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.12 recombinant His-tagged PilB forms in Escherichia coli strain Xl-1 blue by nickel affinity chromatography Neisseria gonorrhoeae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.11 additional information the bifunctional enzyme catalyzes both reactions of MsrB or PilB, EC 1.8.4.12, and of MsrA or PilA, EC 1.8.4.11, the catalytic sites for the two different activities are localized separately on the enzyme molecule, overview Neisseria gonorrhoeae ?
-
 ?
1.8.4.11 additional information the bifunctional enzyme catalyzes both reactions of MsrB or PilB, EC 1.8.4.12, and of MsrA or PilA, EC 1.8.4.11, the catalytic sites for the two different activities are localized separately on the enzyme molecule, overview Neisseria gonorrhoeae MS11 ?
-
 ?
1.8.4.12 additional information PilB affects the survival of the organism to reactive oxygen species, PilB is not involved in piliation, pilin production, or adherence Neisseria gonorrhoeae ?
-
 ?
1.8.4.12 additional information the bifunctional enzyme catalyzes both reactions of MsrB or PilB, EC 1.8.4.12, and of MsrA or PilA, EC 1.8.4.11, the catalytic sites for the two different activities are localized separatly on the enzyme molecule, overview Neisseria gonorrhoeae ?
-
 ?
1.8.4.12 additional information PilB affects the survival of the organism to reactive oxygen species, PilB is not involved in piliation, pilin production, or adherence Neisseria gonorrhoeae MS11 ?
-
 ?
1.8.4.12 additional information the bifunctional enzyme catalyzes both reactions of MsrB or PilB, EC 1.8.4.12, and of MsrA or PilA, EC 1.8.4.11, the catalytic sites for the two different activities are localized separatly on the enzyme molecule, overview Neisseria gonorrhoeae MS11 ?
-
 ?
1.8.4.12 N-acetyl-L-methionine-(R)-S-oxide + thioredoxin
-
 Neisseria gonorrhoeae N-acetyl-L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.12 N-acetyl-L-methionine-(R)-S-oxide + thioredoxin
-
 Neisseria gonorrhoeae MS11 N-acetyl-L-methionine + thioredoxin disulfide + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.4.12 ? x * 15000-35000, recombinant His-tagged PilB forms, SDS-PAGE Neisseria gonorrhoeae

Synonyms

EC Number Synonyms Comment Organism
1.8.4.11 MsrA
-
 Neisseria gonorrhoeae
1.8.4.11 PilA
-
 Neisseria gonorrhoeae
1.8.4.12 MsrB
-
 Neisseria gonorrhoeae
1.8.4.12 PilB
-
 Neisseria gonorrhoeae

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.12 thioredoxin
-
 Neisseria gonorrhoeae