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Literature summary extracted from

  • Seiffert, G.B.; Ullmann, G.M.; Messerschmidt, A.; Schink, B.; Kroneck, P.M.H.; Einsle, O.
    Structure of the non-redox-active tungsten/[4Fe:4S] enzyme acetylene hydratase (2007), Proc. Natl. Acad. Sci. USA, 104, 3073-3077.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.112 purified enzyme, the mother liquor contains plus 15% (v/v) 2-methyl-2,5-pentanediol, X-ray diffraction structure determination and analysis at 1.26-1.95 A resolution, modeling Syntrophotalea acetylenica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.112 Iron non-redox-active tungsten/[4Fe-4S] enzyme, a cubane-type [4Fe:4S] cluster, structure analysis, overview Syntrophotalea acetylenica
4.2.1.112 Tungsten a non-redox-active tungsten/[4Fe-4S] enzyme, contains two molybdopterin guanine dinucleotide cofactors, MGD, designated P and Q, structure analysis, bis-molybdopterin guanine dinucleotide-ligated tungsten atom, overview, the tungsten center binds a water molecule that is activated by an adjacent aspartate residue, enabling to attack acetylene bound in a distinct, hydrophobic pocket, this mechanism requires a strong shift of pKa of the aspartate, caused by nearby low-potential [4Fe:4S] cluster, overview Syntrophotalea acetylenica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.112 acetylene + H2O Syntrophotalea acetylenica
-
 acetaldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.112 Syntrophotalea acetylenica
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.112 acetaldehyde = acetylene + H2O active site channel structure and detailed catalytic mechanism Syntrophotalea acetylenica
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.112 additional information strictly anaerobic growth conditions Syntrophotalea acetylenica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.112 acetylene + H2O
-
 Syntrophotalea acetylenica acetaldehyde
-
 ?
4.2.1.112 acetylene + H2O a non-redox reaction Syntrophotalea acetylenica acetaldehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.112 More four domain structure with active site access pathway, active site architecture, overview Syntrophotalea acetylenica

Synonyms

EC Number Synonyms Comment Organism
4.2.1.112 More the enzyme structurally belongs to the dimethyl sulfoxide reductase family of enzymes Syntrophotalea acetylenica