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Literature summary extracted from

  • Gross, E.; Sevier, C.S.; Heldman, N.; Vitu, E.; Bentzur, M.; Kaiser, C.A.; Thorpe, C.; Fass, D.
    Generating disulfides enzymatically: reaction products and electron acceptors of the endoplasmic reticulum thiol oxidase Ero1p (2006), Proc. Natl. Acad. Sci. USA, 103, 299-304.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.3.2 expressed in Escherichia coli strain B(DE3)pLysS Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.3.2 0.0001
-
 O2 with 0.3 mM reduced thioredoxin as the substrate of the reductive half-reaction and an initial oxygen concentration of around 0.3 mM such that the reduced thioredoxin would be depleted by 10% during the course of the reaction Saccharomyces cerevisiae
1.8.3.2 0.0004
-
 O2 in the presence of 12.5 mM dithiothreitol Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.3.2 48660
-
 method not mentioned Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.2 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.3.2 glutathione-Sepharose column Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.2 2 R-SH + FAD
-
 Saccharomyces cerevisiae R-S-S-R + FADH2
-
 ?
1.8.3.2 dithiothreitol + O2
-
 Saccharomyces cerevisiae dithiothreitol disulfide + H2O2
-
 ?
1.8.3.2 R-SH + O2
-
 Saccharomyces cerevisiae R-S-S-R + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.3.2 Ero1p
-
 Saccharomyces cerevisiae