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Literature summary extracted from

  • Hayashi, M.; Takahashi, H.; Tamura, K.; Huang, J.; Yu, L.H.; Kawai-Yamada, M.; Tezuka, T.; Uchimiya, H.
    Enhanced dihydroflavonol-4-reductase activity and NAD homeostasis leading to cell death tolerance in transgenic rice (2005), Proc. Natl. Acad. Sci. USA, 102, 7020-7025.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.219 Oryza sativa
-
 Oryza sativa L. cv. Nipponbare
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.219 callus
-
 Oryza sativa
-
1.1.1.219 plant
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.219 0.042
-
 sample callus control in 25 mM Tris-HCl (pH 7.0), 4 mM NADPH and 0.1 mM dihydroquercetin at 25°C Oryza sativa
1.1.1.219 0.103
-
 sample callus L-1 in 25 mM Tris-HCl (pH 7.0), 4 mM NADPH and 0.1 mM dihydroquercetin at 25°C Oryza sativa
1.1.1.219 0.155
-
 sample callus L-2 in 25 mM Tris-HCl (pH 7.0), 4 mM NADPH and 0.1 mM dihydroquercetin at 25°C Oryza sativa
1.1.1.219 0.202
-
 sample plant control in 25 mM Tris-HCl (pH 7.0), 4 mM NADPH and 0.1 mM dihydroquercetin at 25°C Oryza sativa
1.1.1.219 0.268
-
 sample plant L-2 in 25 mM Tris-HCl (pH 7.0), 4 mM NADPH and 0.1 mM dihydroquercetin at 25°C Oryza sativa
1.1.1.219 0.349
-
 sample plant L-1 in 25 mM Tris-HCl (pH 7.0), 4 mM NADPH and 0.1 mM dihydroquercetin at 25°C Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.219 dihydroquercetin + NADPH
-
 Oryza sativa cis-3,4-leucocyanidin + NADP+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.219 30
-
-
 Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.219 7
-
-
 Oryza sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.219 6 7.5
-
 Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.219 NADPH
-
 Oryza sativa