EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.4.12 | expression of wild-type and mutant isozyme MsrB3 in Escherichia coli strain BL21(DE3) | Homo sapiens |
1.8.4.12 | expression of wild-type and mutant isozymes MsrB1 and MsrB2 in Escherichia coli strain BL21(DE3) and in NIH-3T3 cells | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.4.12 | E81V | site-directed mutagenesis, mutation in the selenocysteine-containing or the cysteine-containing isozyme MsrB1, both mutants show reduced activity with either cofactor thioredoxin and DTT compared to wild-type MsrB1s | Mus musculus |
1.8.4.12 | F97N | site-directed mutagenesis, mutation in the selenocysteine-containing or the cysteine-containing isozyme MsrB1, both mutants show altered activity and kinetics compared to wild-type MsrB1s | Mus musculus |
1.8.4.12 | G77H | site-directed mutagenesis, mutation in the selenocysteine-containing or the cysteine-containing isozyme MsrB1, the selenocysteine-containing mutant shows reduced activity with either cofactor thioredoxin and DTT compared to wild-type selenocysteine MsrB1, while the cysteine-containing mutant shows activity and kinetics similar to the wild-type cysteine MsrB1 | Mus musculus |
1.8.4.12 | G77H/E81V/F97N | site-directed mutagenesis, mutation in the selenocysteine-containing or the cysteine-containing isozyme MsrB1, both mutants show altered activity and kinetics compared to wild-type MsrB1s | Mus musculus |
1.8.4.12 | G77H/F97N | site-directed mutagenesis, mutation in the selenocysteine-containing or the cysteine-containing isozyme MsrB1, both mutants show altered activity and kinetics compared to wild-type MsrB1s | Mus musculus |
1.8.4.12 | H77G | site-directed mutagenesis, mutation of isozyme MsrB2 leads to highly reduced activity with either cofactor thioredoxin and DTT compared to wild-type MsrB2 | Mus musculus |
1.8.4.12 | H77G | site-directed mutagenesis, mutation of isozyme MsrB3 leads to highly reduced activity with cofactor thioredoxin or DTT compared to wild-type MsrB3 | Homo sapiens |
1.8.4.12 | H77G/I81E/N97F | site-directed mutagenesis, mutation of isozyme MsrB3, inactive mutant | Homo sapiens |
1.8.4.12 | H77G/N97F | site-directed mutagenesis, mutation of isozyme MsrB2, inactive mutant | Mus musculus |
1.8.4.12 | H77G/N97F | site-directed mutagenesis, mutation of isozyme MsrB3, inactive mutant | Homo sapiens |
1.8.4.12 | H77G/V81E/N97F | site-directed mutagenesis, mutation of isozyme MsrB2, inactive mutant | Mus musculus |
1.8.4.12 | I81E | site-directed mutagenesis, mutation of isozyme MsrB3 leads to slightly increased activity with cofactor thioredoxin and reduced activcity with DTT compared to wild-type MsrB3 | Homo sapiens |
1.8.4.12 | additional information | substitution of Cys residues abolish the enzyme's activity with thioredoxin and increase the DTT-dependent activity, overview | Mus musculus |
1.8.4.12 | additional information | substitution of Cys residues abolish the enzyme's activity with thioredoxin and increase the DTT-dependent activity, overview | Homo sapiens |
1.8.4.12 | N97F | site-directed mutagenesis, mutation of isozyme MsrB2, the mutant is inactive with cofactor thioredoxin and shows highly reduced activity with cofactor DTT compared to wild-type MsrB2 | Mus musculus |
1.8.4.12 | N97F | site-directed mutagenesis, mutation of isozyme MsrB3, the mutant is inactive with cofactor thioredoxin and shows highly reduced activity with cofactor DTT compared to wild-type MsrB3 | Homo sapiens |
1.8.4.12 | N97Y | site-directed mutagenesis, mutation of isozyme MsrB2, the mutant is inactive with cofactor thioredoxin and shows highly reduced activity with cofactor DTT compared to wild-type MsrB2 | Mus musculus |
1.8.4.12 | N97Y | site-directed mutagenesis, mutation of isozyme MsrB3, the mutant shows highly reduced activity with cofactor DTT or thioredoxin compared to wild-type MsrB3 | Homo sapiens |
1.8.4.12 | V81E | site-directed mutagenesis, mutation of isozyme MsrB2 leads to reduced activity with either cofactor thioredoxin and DTT compared to wild-type MsrB2 | Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.12 | additional information | - |
additional information | thioredoxin- and DTT-dependent kinetics of wild-type and mutant enzymes, overview | Mus musculus | |
1.8.4.12 | additional information | - |
additional information | thioredoxin- and DTT-dependent kinetics of wild-type and mutant enzymes, overview | Homo sapiens | |
1.8.4.12 | 0.31 | - |
L-methionine-(R)-S-oxide | recombinant wild-type isozyme MsrB2 expressed in Escherichia coli | Mus musculus | |
1.8.4.12 | 0.5 | - |
L-methionine-(R)-S-oxide | recombinant wild-type selenocysteine-containing isozyme MsrB1 expressed in NIH 3T3 cells | Mus musculus | |
1.8.4.12 | 0.8 | - |
L-methionine-(R)-S-oxide | recombinant wild-type isozyme MsrB3 expressed in Escherichia coli | Homo sapiens | |
1.8.4.12 | 1.3 | - |
L-methionine-(R)-S-oxide | recombinant wild-type cysteine-containing isozyme MsrB1 expressed in NIH 3T3 cells | Mus musculus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.8.4.12 | additional information | isozyme MsrB1 contains selenocysteine, while isozymes MsrB2 and MsrB3 contain cysteine residues, the thioredoxin dependence is different for selenocysteine- and cysteine-containing enzyme, overview | Mus musculus | |
1.8.4.12 | additional information | native isozyme MsrB1 contains selenocysteine, while native isozymes MsrB2 and MsrB3 contain cysteine residues, the thioredoxin dependence is different for selenocysteine- and cysteine-containing enzyme, overview | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.12 | L-methionine-(R)-S-oxide + thioredoxin | Mus musculus | - |
L-methionine + thioredoxin disulfide + H2O | - |
? | |
1.8.4.12 | L-methionine-(R)-S-oxide + thioredoxin | Homo sapiens | - |
L-methionine + thioredoxin disulfide + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.4.12 | Homo sapiens | - |
isozymes MsrB1, MsrB2, and MsrB3 | - |
1.8.4.12 | Mus musculus | - |
isozymes MsrB1, MsrB2, and MsrB3 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.4.12 | recombinant wild-type and mutant isozyme MsrB3 from Escherichia coli strain BL21(DE3) | Homo sapiens |
1.8.4.12 | recombinant wild-type and mutant isozymes MsrB1 and MsrB2 from Escherichia coli strain BL21(DE3) and NIH-3T3 cells | Mus musculus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.8.4.12 | peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (R)-S-oxide + thioredoxin | catalytic mechanism involving residues at positions 95, 41, 97, 77, and 80, molecular modeling, role of selenocysteine- and cysteine residues in catalysis, overview | Mus musculus | |
1.8.4.12 | peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (R)-S-oxide + thioredoxin | catalytic mechanism involving residues at positions 95, 41, 97, 77, and 80, molecular modeling, role of selenocysteine- and cysteine residues in catalysis, overview | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.8.4.12 | additional information | - |
thioredoxin- and DTT-dependent activities of mutant enzymes, overview | Mus musculus |
1.8.4.12 | additional information | - |
thioredoxin- and DTT-dependent activities of mutant enzymes, overview | Homo sapiens |
1.8.4.12 | 0.000046 | - |
purified recombinant wild-type cysteine-containing isozyme MsrB1 expressed in NIH 3T3 cells, substrates are L-methionine-(R)-S-oxide and thioredoxin | Mus musculus |
1.8.4.12 | 0.002 | - |
purified recombinant wild-type cysteine-containing isozyme MsrB1 expressed in NIH 3T3 cells, substrates are L-methionine-(R)-S-oxide and DTT | Mus musculus |
1.8.4.12 | 0.01 | - |
purified recombinant wild-type isozyme MsrB2 expressed in Escherichia coli, substrates are L-methionine-(R)-S-oxide and thioredoxin | Mus musculus |
1.8.4.12 | 0.023 | - |
purified recombinant wild-type isozyme MsrB3 expressed in Escherichia coli, substrates are L-methionine-(R)-S-oxide and thioredoxin | Homo sapiens |
1.8.4.12 | 0.045 | - |
purified recombinant wild-type selenocysteine-containing isozyme MsrB1 expressed in NIH 3T3 cells, substrates are L-methionine-(R)-S-oxide and thioredoxin | Mus musculus |
1.8.4.12 | 0.17 | - |
purified recombinant wild-type selenocysteine-containing isozyme MsrB1 expressed in NIH 3T3 cells, substrates are L-methionine-(R)-S-oxide and DTT | Mus musculus |
1.8.4.12 | 0.386 | - |
purified recombinant wild-type isozyme MsrB2 expressed in Escherichia coli, substrates are L-methionine-(R)-S-oxide and DTT | Mus musculus |
1.8.4.12 | 0.452 | - |
purified recombinant wild-type isozyme MsrB3 expressed in Escherichia coli, substrates are L-methionine-(R)-S-oxide and DTT | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.12 | L-methionine-(R)-S-oxide + DTT | isozymes MsrB1, MsrB2, and MsrB3 | Mus musculus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
1.8.4.12 | L-methionine-(R)-S-oxide + thioredoxin | - |
Mus musculus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
1.8.4.12 | L-methionine-(R)-S-oxide + thioredoxin | - |
Homo sapiens | L-methionine + thioredoxin disulfide + H2O | - |
? | |
1.8.4.12 | L-methionine-(R)-S-oxide + thioredoxin | isozymes MsrB1, MsrB2, and MsrB3 | Mus musculus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
1.8.4.12 | L-methionine-(R)-S-oxide + thioredoxin | isozymes MsrB1, MsrB2, and MsrB3 | Homo sapiens | L-methionine + thioredoxin disulfide + H2O | - |
? | |
1.8.4.12 | additional information | the thioredoxin dependence is different for selenocysteine- and cysteine-containing enzyme, overview | Mus musculus | ? | - |
? | |
1.8.4.12 | additional information | the thioredoxin dependence is different for selenocysteine- and cysteine-containing enzyme, overview | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.4.12 | cysteine-containing methionine-R-sulfoxide reductase | - |
Mus musculus |
1.8.4.12 | cysteine-containing methionine-R-sulfoxide reductase | - |
Homo sapiens |
1.8.4.12 | methionine sulfoxide reductase B | - |
Mus musculus |
1.8.4.12 | methionine sulfoxide reductase B | - |
Homo sapiens |
1.8.4.12 | MsrB | - |
Mus musculus |
1.8.4.12 | MsrB | - |
Homo sapiens |
1.8.4.12 | selenocysteine-containing methionine-R-sulfoxide reductase | - |
Mus musculus |
1.8.4.12 | selenocysteine-containing methionine-R-sulfoxide reductase | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.12 | 0.002 | - |
L-methionine-(R)-S-oxide | recombinant wild-type cysteine-containing isozyme MsrB1 expressed in NIH 3T3 cells | Mus musculus | |
1.8.4.12 | 0.22 | - |
L-methionine-(R)-S-oxide | recombinant wild-type selenocysteine-containing isozyme MsrB1 expressed in NIH 3T3 cells | Mus musculus | |
1.8.4.12 | 0.46 | - |
L-methionine-(R)-S-oxide | recombinant wild-type isozyme MsrB2 expressed in Escherichia coli | Mus musculus | |
1.8.4.12 | 0.83 | - |
L-methionine-(R)-S-oxide | recombinant wild-type isozyme MsrB3 expressed in Escherichia coli | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.4.12 | dithiothreitol | results in higher activity compared to cofactor thioredoxin | Mus musculus | |
1.8.4.12 | thioredoxin | - |
Mus musculus |