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Literature summary extracted from
- A conserved mechanism for sulfonucleotide reduction (2005), PLoS Biol., 3, 1418-1435.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.99.2 | expressed in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.99.2 | C249S | unreactive toward 4-vinylpyridine labelling | Mycobacterium tuberculosis |
| 1.8.99.2 | C59S | unreactive toward 4-vinylpyridine labelling | Mycobacterium tuberculosis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.2 | 28513 | - |
1 * 28513, analytical ultracentrifugation | Mycobacterium tuberculosis |
| 1.8.99.2 | 28560 | - |
calculated from amino acid sequence | Mycobacterium tuberculosis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.99.2 | Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.99.2 | HiTrap chelating column chromatography | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.2 | 4.6 | - |
untagged APS reductase using Escherichia coli thioredoxin | Mycobacterium tuberculosis |
| 1.8.99.2 | 5.1 | - |
His-tagged APS reductase using Escherichia coli thioredoxin | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.99.2 | adenylyl sulfate + reduced thioredoxin | - |
Mycobacterium tuberculosis | AMP + sulfite + oxidized thioredoxin | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.99.2 | monomer | 1 * 28513, analytical ultracentrifugation | Mycobacterium tuberculosis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.99.2 | adenosine-5'-phosphosulfate reductase | - |
Mycobacterium tuberculosis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.2 | thioredoxin | - |
Mycobacterium tuberculosis | |
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Release 2023.1 (January 2023)
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