Literature summary extracted from
Miller, B.; Oschinski, C.; Zimmer, W.
First isolation of an isoprene synthase gene from poplar and successful expression of the gene in Escherichia coli (2001), Planta, 213, 483-487.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.2.3.27 |
gene ispS, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His-tagged enzyme with or without leader sequence in Escherichia coli strain TG1 |
Populus tremula x Populus alba |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
4.2.3.27 |
chloroplast |
the enzyme contains a plastidial leader sequence |
Populus tremula x Populus alba |
9507 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.2.3.27 |
Mg2+ |
dependent on, the enzyme contains the Mg2+-binding DDXXD motif, typical for monoterpene synthases |
Populus tremula x Populus alba |
|
4.2.3.27 |
Mn2+ |
can substitute for Mg2+ |
Populus tremula x Populus alba |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.2.3.27 |
dimethylallyl diphosphate |
Populus tremula x Populus alba |
- |
isoprene + diphosphate |
isoprene is a volatile product emmitted to the atmosphere |
ir |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.2.3.27 |
Populus tremula x Populus alba |
Q9AR86 |
precursor |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
4.2.3.27 |
proteolytic modification |
the enzyme contains a plastidial leader sequence |
Populus tremula x Populus alba |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.2.3.27 |
recombinant His-tagged enzyme from Escherichia coli strain TG1 |
Populus tremula x Populus alba |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
4.2.3.27 |
0.012 |
0.156 |
purified recombinant enzyme with or without leader sequence |
Populus tremula x Populus alba |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.2.3.27 |
dimethylallyl diphosphate |
- |
Populus tremula x Populus alba |
isoprene + diphosphate |
isoprene is a volatile product emmitted to the atmosphere |
ir |
|
4.2.3.27 |
dimethylallyl diphosphate |
- |
Populus tremula x Populus alba |
isoprene + diphosphate |
i.e. 2-methyl-1,3-butadiene |
ir |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.2.3.27 |
More |
the enzyme contains the Mg2+-binding DDXXD motif, typical for monoterpene synthases |
Populus tremula x Populus alba |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
4.2.3.27 |
40 |
- |
assay at |
Populus tremula x Populus alba |