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Literature summary extracted from

  • Lee, Y.; Ta, H.T.; Duggleby, R.G.
    Cyclopropane-1,1-dicarboxylate is a slow-, tight-binding inhibitor of rice ketol-acid reductoisomerase (2005), Plant Sci., 168, 1035-1040.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.86 expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.86 1-aminocarbonylcyclopropanecarboxylate
-
 Oryza sativa
1.1.1.86 1-cyanocyclopropanecarboxylate
-
 Oryza sativa
1.1.1.86 1-hydroxycyclopropanecarboxylate
-
 Oryza sativa
1.1.1.86 cyclopropane-1,1-dicarboxylate a competitive slow-, tight-binding inhibitor Oryza sativa
1.1.1.86 dimethylmalonate slow-binding inhibitor Oryza sativa
1.1.1.86 additional information no inhibition by 1-aminocyclopropanecarboxylate, 1-methylcyclopropanecarboxylate, 2-methylcyclopropanecarboxylate, cyclopropane-1,2-dicarboxylate, and cyclopropanecarboxylate dimethyl ester Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.86 0.0072
-
 NADPH pH 8.0, 30°C, recombinant enzyme Oryza sativa
1.1.1.86 0.042
-
 2-acetolactate pH 8.0, 30°C, recombinant enzyme Oryza sativa
1.1.1.86 0.893
-
 Hydroxypyruvate pH 8.0, 30°C, recombinant enzyme Oryza sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.86 Mg2+ required, KM: 0.00324 mM Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.86 Oryza sativa Q65XK0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.86 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography Oryza sativa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.86 1.4
-
 purified recombinant enzyme, substrate 2-acetolactate Oryza sativa
1.1.1.86 1.97
-
 purified recombinant enzyme, substrate hydroxypyruvate Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.86 2-acetolactate + NADPH
-
 Oryza sativa 2,3-dihydroxyisovalerate + NADP+
-
 ?
1.1.1.86 hydroxypyruvate + NADPH
-
 Oryza sativa glycerate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.86 KARI
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.86 30
-
 assay at Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.86 8
-
 assay at Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.86 NADPH
-
 Oryza sativa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.86 additional information
-
 additional information inhibition mechanism and kinetics Oryza sativa
1.1.1.86 0.0000903
-
 cyclopropane-1,1-dicarboxylate pH 8.0, 30°C, recombinant enzyme, versus hydroxypyruvate Oryza sativa
1.1.1.86 0.000716
-
 dimethylmalonate pH 8.0, 30°C, recombinant enzyme Oryza sativa
1.1.1.86 0.0056
-
 1-hydroxycyclopropanecarboxylate pH 8.0, 30°C, recombinant enzyme Oryza sativa
1.1.1.86 0.0203
-
 1-aminocarbonylcyclopropanecarboxylate pH 8.0, 30°C, recombinant enzyme Oryza sativa
1.1.1.86 0.0585
-
 1-cyanocyclopropanecarboxylate pH 8.0, 30°C, recombinant enzyme Oryza sativa