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Literature summary extracted from

  • Vieira Dos Santos, C.; Cuine, S.; Rouhier, N.; Rey, P.
    The Arabidopsis plastidic methionine sulfoxide reductase B proteins. Sequence and activity characteristics, comparison of the expression with plastidic methionine sulfoxide reductase A, and induction by photooxidative stress (2005), Plant Physiol., 138, 909-922.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.12 DNA and amino acid sequence determination and analysis of plastidic isozymes MsrB1 and MsrB2, functional expression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.12 0.054
-
 L-methionine-(R)-S-oxide recombinant isozyme MsrB2, with dithioerythritol Arabidopsis thaliana
1.8.4.12 0.079
-
 L-methionine-(R)-S-oxide recombinant isozyme MsrB1, with dithioerythritol Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.12 chloroplast plastidic isozymes MsrB1 and MsrB2, analysis of subcellular localization Arabidopsis thaliana 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.12 L-methionine-(R)-S-oxide + thioredoxin Arabidopsis thaliana absolute stereospecific reduction L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.12 Arabidopsis thaliana
-
 plastidic isozymes MsrB1 and MsrB2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.12 recombinant plastidic isozymes MsrB1 and MsrB2 from Escherichia coli Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.4.12 leaf
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.4.12 additional information
-
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.12 L-methionine-(R)-S-oxide + dithioerythritol absolute stereospecific reduction, MsrB1 and MsrB2 Arabidopsis thaliana L-methionine + dithioerythritol disulfide + H2O
-
 ?
1.8.4.12 L-methionine-(R)-S-oxide + thioredoxin absolute stereospecific reduction Arabidopsis thaliana L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.12 L-methionine-(R)-S-oxide + thioredoxin absolute stereospecific reduction, isozyme MsrB2, no activity with isozyme MsrB1 Arabidopsis thaliana L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.12 additional information substrate specificity, overview, isozyme MsrB1 is not able to reduce free L-methionine-(R)-S-oxide or N-acetyl-L-methionine-(R)-S-oxide, while isozyme MsrB2 prefers protein-bound substrates such as N-acetyl-L-methionine (R)-S-oxide, overview Arabidopsis thaliana ?
-
 ?
1.8.4.12 N-acetyl-L-methionine-(R)-S-oxide + thioredoxin protein-bound substrate, preferred substrate of isozyme MsrB2 Arabidopsis thaliana N-acetyl-L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.12 methionine sulfoxide reductase B
-
 Arabidopsis thaliana
1.8.4.12 MsrB
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.4.12 0.02
-
 L-methionine-(R)-S-oxide recombinant isozyme MsrB2, with dithioerythritol Arabidopsis thaliana
1.8.4.12 0.07
-
 L-methionine-(R)-S-oxide recombinant isozyme MsrB1, with dithioerythritol Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.12 dithioerythritol DTE Arabidopsis thaliana
1.8.4.12 thioredoxin isozyme MsrB2, no activity with isozyme MsrB1 Arabidopsis thaliana