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Literature summary extracted from

  • Voegele, R.T.; Hahn, M.; Lohaus, G.; Link, T.; Heiser, I.; Mendgen, K.
    Possible roles for mannitol and mannitol dehydrogenase in the biotrophic plant pathogen Uromyces fabae (2005), Plant Physiol., 137, 190-198.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.138 gene MAD1, expression analysis in fungal tissue, recombinant expression in yeast Uromyces viciae-fabae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.138 additional information
-
 additional information kinetic and thermodynamic analysis, recombinant enzyme Uromyces viciae-fabae
1.1.1.138 0.034
-
 NADP+ pH 10.0, recombinant enzyme Uromyces viciae-fabae
1.1.1.138 0.038
-
 NADPH pH 5.2, recombinant enzyme Uromyces viciae-fabae
1.1.1.138 0.804
-
 D-fructose pH 5.2, recombinant enzyme Uromyces viciae-fabae
1.1.1.138 78
-
 D-mannitol pH 10.0, recombinant enzyme Uromyces viciae-fabae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.138 D-mannitol + NADP+ Uromyces viciae-fabae the enzyme has a dual role in mannitol metabolism, D-mannitol is the main storage sugar in spores D-fructose + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.138 Uromyces viciae-fabae
-
 biotrophic plant pathogenic rust fungus, gene MAD1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.138 haustorial mother cell
-
 Uromyces viciae-fabae
-
1.1.1.138 haustorium lumen Uromyces viciae-fabae
-
1.1.1.138 additional information no activity in intercellular hyphae, the pathogen infects leaves of Vicia faba forming haustoria Uromyces viciae-fabae
-
1.1.1.138 spore
-
 Uromyces viciae-fabae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.138 0.4
-
 recombinant enzyme, forward reaction Uromyces viciae-fabae
1.1.1.138 1
-
 recombinant enzyme, reverse reaction Uromyces viciae-fabae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.138 D-mannitol + NADP+ the enzyme has a dual role in mannitol metabolism, D-mannitol is the main storage sugar in spores Uromyces viciae-fabae D-fructose + NADPH + H+
-
 r
1.1.1.138 D-mannitol + NADP+ the reverse reaction occurs only when D-fructose is provided as substrate, and is preferred Uromyces viciae-fabae D-fructose + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.138 MAD1p
-
 Uromyces viciae-fabae
1.1.1.138 mannitol dehydrogenase 1
-
 Uromyces viciae-fabae
1.1.1.138 PIG8p formerly Uromyces viciae-fabae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.138 6
-
 reverse reaction, recombinant enzyme Uromyces viciae-fabae
1.1.1.138 10.5
-
 forward reaction, recombinant enzyme Uromyces viciae-fabae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.138 8.5 11 sharp decline below pH 8.5 and above pH 11.0, forward reaction, recombinant enzyme Uromyces viciae-fabae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.138 NADP+
-
 Uromyces viciae-fabae
1.1.1.138 NADPH
-
 Uromyces viciae-fabae