Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kato, M.; Mizuno, K.; Fujimura, T.; Iwama, M.; Irie, M.; Crozier, A.; Ashihara, H.
    Purification and characterization of caffeine synthase from tea leaves (1999), Plant Physiol., 120, 579-586.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.159 41000
-
 x * 41000, SDS-PAGE Camellia sinensis
2.1.1.159 61000
-
 gel filtration Camellia sinensis
2.1.1.160 41000
-
 x * 41000, SDS-PAGE Camellia sinensis
2.1.1.160 61000
-
 gel filtration Camellia sinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.159 additional information Camellia sinensis the bifunctional enzyme catalyzes the last two steps of the caffeine biosynthesis ?
-
 ?
2.1.1.159 S-adenosyl-L-methionine + 3,7-dimethylxanthine Camellia sinensis reaction of EC 2.1.1.160 S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine Camellia sinensis
-
 S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?
2.1.1.160 additional information Camellia sinensis the bifunctional enzyme catalyzes the last two steps of the caffeine biosynthesis ?
-
 ?
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine Camellia sinensis
-
 S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.159 Camellia sinensis
-
 bifunctional enzyme
-
2.1.1.160 Camellia sinensis
-
 bifunctional enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.159 native enzyme 523fold from young, developing leaves by ammonium sulfate fractionation, hydroxylapatite, anion exchange, and adenosine affinity chromatography, and gel filtration to homogeneity Camellia sinensis
2.1.1.160 native enzyme 523fold from young, developing leaves by ammonium sulfate fractionation, hydroxylapatite, anion exchange, and adenosine affinity chromatography, and gel filtration to homogeneity Camellia sinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.159 leaf young, developing Camellia sinensis
-
2.1.1.160 leaf young, developing Camellia sinensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.159 95
-
 purified enzyme Camellia sinensis
2.1.1.160 95
-
 purified enzyme Camellia sinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.159 additional information the bifunctional enzyme catalyzes the last two steps of the caffeine biosynthesis Camellia sinensis ?
-
 ?
2.1.1.159 additional information substrate specificity, overview, the bifunctional enzyme also catalyzes the reaction of the caffeine synthase, EC 2.1.1.160, no activity with xanthosine, xanthosine 5-phosphate, and theophylline Camellia sinensis ?
-
 ?
2.1.1.159 S-adenosyl-L-methionine + 1-methylxanthine low activity Camellia sinensis S-adenosyl-L-homocysteine + 1,3-dimethylxanthine i.e. theophylline ?
2.1.1.159 S-adenosyl-L-methionine + 3,7-dimethylxanthine reaction of EC 2.1.1.160 Camellia sinensis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
2.1.1.159 S-adenosyl-L-methionine + 3-methylxanthine low activity Camellia sinensis S-adenosyl-L-homocysteine + 1,3-dimethylxanthine i.e. theophylline ?
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine
-
 Camellia sinensis S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?
2.1.1.159 S-adenosyl-L-methionine + paraxanthine best substrate Camellia sinensis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
2.1.1.160 additional information the bifunctional enzyme catalyzes the last two steps of the caffeine biosynthesis Camellia sinensis ?
-
 ?
2.1.1.160 additional information substrate specificity, overview, the bifunctional enzyme mainly catalyzes the reaction of the theobromine synthase, EC 2.1.1.159, no activity with xanthosine, xanthosine 5-phosphate, and theophylline Camellia sinensis ?
-
 ?
2.1.1.160 S-adenosyl-L-methionine + 1-methylxanthine low activity Camellia sinensis S-adenosyl-L-homocysteine + 1,3-dimethylxanthine i.e. theophylline ?
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
 Camellia sinensis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
2.1.1.160 S-adenosyl-L-methionine + 3-methylxanthine low activity Camellia sinensis S-adenosyl-L-homocysteine + 1,3-dimethylxanthine i.e. theophylline ?

Subunits

EC Number Subunits Comment Organism
2.1.1.159 monomer or dimer x * 41000, SDS-PAGE Camellia sinensis
2.1.1.160 monomer or dimer x * 41000, SDS-PAGE Camellia sinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.159 8.5
-
 sharp optimum Camellia sinensis
2.1.1.160 8.5
-
 sharp optimum Camellia sinensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.159 additional information
-
 pH profile Camellia sinensis
2.1.1.160 additional information
-
 pH profile Camellia sinensis