Literature summary extracted from
Kuzma, J.; Fall, R.
Leaf isoprene emission rate is dependent on leaf development and the level of isoprene synthase (1993), Plant Physiol., 101, 435-440.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
4.2.3.27 |
additional information |
the enzyme is positively regulated by light |
Mucuna sp. |
|
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.2.3.27 |
Ca2+ |
stimulates at up to 2 mM, inhibits at 2-30 mM |
Mucuna sp. |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.2.3.27 |
Ca2+ |
stimulates at up to 2 mM, inhibits at 2-30 mM |
Mucuna sp. |
|
4.2.3.27 |
Mg2+ |
dependent on, best at 15 mM, 20fold higher activity compared to Mn2+ |
Mucuna sp. |
|
4.2.3.27 |
Mn2+ |
can partially substitute for Mg2+, 20fold lower activity compared to Mg2+ |
Mucuna sp. |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.2.3.27 |
dimethylallyl diphosphate |
Mucuna sp. |
- |
isoprene + diphosphate |
isoprene is emmitted to the atmosphere |
ir |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.2.3.27 |
Mucuna sp. |
- |
velvet bean |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.2.3.27 |
native enzyme partially by ammonium sulfate fractionation and anion exchange chromatography |
Mucuna sp. |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
4.2.3.27 |
leaf |
activity during leaf development, highest activity an isoprene emission in about 14-days-old leaves, overview |
Mucuna sp. |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
4.2.3.27 |
additional information |
- |
activity and isoprene emission during leaf development, overview |
Mucuna sp. |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.2.3.27 |
dimethylallyl diphosphate |
- |
Mucuna sp. |
isoprene + diphosphate |
isoprene is emmitted to the atmosphere |
ir |
|
4.2.3.27 |
dimethylallyl diphosphate |
- |
Mucuna sp. |
isoprene + diphosphate |
i.e. 2-methyl-1,3-butadiene |
ir |
|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
4.2.3.27 |
30 |
- |
assay at |
Mucuna sp. |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
4.2.3.27 |
7.8 |
8.5 |
- |
Mucuna sp. |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
4.2.3.27 |
5.4 |
11.2 |
- |
Mucuna sp. |