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Literature summary extracted from
- Tropinone reductases, enzymes at the branch point of tropane alkaloid metabolism (2006), Phytochemistry, 67, 327-337.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.236 | expressed in Escherichia coli | Solanum tuberosum |
| 1.1.1.236 | expressed in Escherichia coli | Datura stramonium |
| 1.1.1.236 | expressed in Escherichia coli | Atropa belladonna |
| 1.1.1.236 | expressed in Escherichia coli | Hyoscyamus niger |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.206 | 0.0113 | - |
NADPH | - |
Hyoscyamus niger |  |
| 1.1.1.206 | 0.0415 | - |
NADP+ | - |
Hyoscyamus niger | |
| 1.1.1.206 | 0.058 | - |
NADPH | - |
Datura stramonium | |
| 1.1.1.206 | 0.105 | - |
NADP+ | - |
Datura stramonium | |
| 1.1.1.206 | 0.775 | - |
tropinone | pH 5.9 | Datura stramonium | |
| 1.1.1.206 | 1.01 | - |
tropinone | pH 5.9 | Hyoscyamus niger | |
| 1.1.1.206 | 1.3 | - |
tropinone | pH 6.4 | Datura stramonium | |
| 1.1.1.236 | 0.002 | - |
NADPH | - |
Solanum tuberosum | |
| 1.1.1.236 | 0.0021 | - |
NADPH | - |
Atropa belladonna | |
| 1.1.1.236 | 0.0061 | - |
NADPH | - |
Hyoscyamus niger | |
| 1.1.1.236 | 0.016 | - |
NADPH | at pH 5.95 | Datura stramonium | |
| 1.1.1.236 | 0.033 | - |
tropinone | at pH 6.4 | Solanum tuberosum | |
| 1.1.1.236 | 0.033 | - |
tropinone | at pH 5.95 | Datura stramonium | |
| 1.1.1.236 | 0.034 | - |
tropinone | at pH 5.9 | Hyoscyamus niger | |
| 1.1.1.236 | 0.09 | - |
tropinone | at pH 6.25 | Atropa belladonna | |
| 1.1.1.236 | 0.265 | - |
N-propyl-4-piperidinone | - |
Hyoscyamus niger | |
| 1.1.1.236 | 0.38 | - |
4-tetrahydro-thiopyranone | - |
Atropa belladonna | |
| 1.1.1.236 | 0.534 | - |
4-ethyl-cyclohexanone | - |
Hyoscyamus niger | |
| 1.1.1.236 | 0.65 | - |
N-methyl-4-piperidinone | - |
Atropa belladonna | |
| 1.1.1.236 | 0.77 | - |
N-methyl-4-piperidinone | - |
Hyoscyamus niger | |
| 1.1.1.236 | 2 | - |
4-tetrahydro-thiopyranone | - |
Datura stramonium | |
| 1.1.1.236 | 2.03 | - |
4-methyl-cyclohexanone | - |
Hyoscyamus niger | |
| 1.1.1.236 | 2.8 | - |
4-methyl-cyclohexanone | - |
Datura stramonium | |
| 1.1.1.236 | 7.58 | - |
3-methyl-cyclohexanone | - |
Hyoscyamus niger | |
| 1.1.1.236 | 20 | - |
N-methyl-4-piperidinone | - |
Datura stramonium | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.236 | 28310 | - |
calculation from sequence of cDNA | Solanum tuberosum |
| 1.1.1.236 | 28310 | - |
calculation from sequence of cDNA | Datura stramonium |
| 1.1.1.236 | 28310 | - |
calculation from sequence of cDNA | Atropa belladonna |
| 1.1.1.236 | 28310 | - |
calculation from sequence of cDNA | Hyoscyamus niger |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.206 | tropinone + NADPH + H+ | Datura stramonium | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | tropine + NADP+ | - |
r | |
| 1.1.1.206 | tropinone + NADPH + H+ | Atropa belladonna | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | tropine + NADP+ | - |
? | |
| 1.1.1.206 | tropinone + NADPH + H+ | Duboisia myoporoides | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | tropine + NADP+ | - |
? | |
| 1.1.1.206 | tropinone + NADPH + H+ | Duboisia leichhardtii | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | tropine + NADP+ | - |
? | |
| 1.1.1.206 | tropinone + NADPH + H+ | Hyoscyamus niger | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | tropine + NADP+ | - |
r | |
| 1.1.1.206 | tropinone + NADPH + H+ | Hyoscyamus muticus | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | tropine + NADP+ | - |
? | |
| 1.1.1.206 | tropinone + NADPH + H+ | Scopolia atropoides | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | tropine + NADP+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.206 | Atropa belladonna | - |
- |
- |
| 1.1.1.206 | Datura stramonium | - |
- |
- |
| 1.1.1.206 | Duboisia leichhardtii | - |
- |
- |
| 1.1.1.206 | Duboisia myoporoides | - |
- |
- |
| 1.1.1.206 | Hyoscyamus muticus | - |
- |
- |
| 1.1.1.206 | Hyoscyamus niger | - |
- |
- |
| 1.1.1.206 | Scopolia atropoides | - |
- |
- |
| 1.1.1.236 | Atropa belladonna | - |
- |
- |
| 1.1.1.236 | Datura stramonium | - |
- |
- |
| 1.1.1.236 | Hyoscyamus niger | - |
- |
- |
| 1.1.1.236 | Solanum tuberosum | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.236 | root | - |
Solanum tuberosum | - |
| 1.1.1.236 | root | - |
Datura stramonium | - |
| 1.1.1.236 | root | - |
Atropa belladonna | - |
| 1.1.1.236 | root | - |
Hyoscyamus niger | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.236 | additional information | - |
quinuclidinone and 8-thiabicylo[3.2.1]octan-3-one are no substrates of TRII | Solanum tuberosum |
| 1.1.1.236 | additional information | - |
quinuclidinone and 8-thiabicylo[3.2.1]octan-3-one are no substrates of TRII | Datura stramonium |
| 1.1.1.236 | additional information | - |
quinuclidinone and 8-thiabicylo[3.2.1]octan-3-one are no substrates of TRII | Atropa belladonna |
| 1.1.1.236 | additional information | - |
quinuclidinone and 8-thiabicylo[3.2.1]octan-3-one are no substrates of TRII | Hyoscyamus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.206 | 3-methyl-cyclohexanone + NADPH | 85% of the reaction velocity with tropinone | Hyoscyamus niger | 3-methylcyclohexanol + NADP+ | - |
? | |
| 1.1.1.206 | 4-ethylcyclohexanone + NADPH | 107% of the reaction velocity with tropinone | Hyoscyamus niger | 4-ethylcyclohexanol + NADP+ | - |
? | |
| 1.1.1.206 | 4-ethylcyclohexanone + NADPH | 41% of the reaction velocity with tropinone | Datura stramonium | 4-ethylcyclohexanol + NADP+ | - |
? | |
| 1.1.1.206 | 4-tetrahydro-thiopyranone + NADPH | 83% of the reaction velocity with tropinone | Datura stramonium | ? | - |
? | |
| 1.1.1.206 | 8-thiabicyclo[3.2.1]octan-3-one + NADPH | 35% of the reaction velocity with tropinone | Datura stramonium | ? | - |
? | |
| 1.1.1.206 | 8-thiabicyclo[3.2.1]octan-3-one + NADPH | 40% of the reaction velocity with tropinone | Hyoscyamus niger | ? | - |
? | |
| 1.1.1.206 | additional information | no activity with 3-methyl-cyclohexanone | Datura stramonium | ? | - |
? | |
| 1.1.1.206 | additional information | no activity with N-propyl-4-pieridinone and 4-tetrahydro-thiopyranone | Hyoscyamus niger | ? | - |
? | |
| 1.1.1.206 | N-methyl-4-piperidinone + NADPH | 180% of the reaction velocity with tropinone | Datura stramonium | N-methyl-1-piperidinol + NADP+ | - |
? | |
| 1.1.1.206 | N-methyl-4-piperidinone + NADPH + H+ | 13% of the reaction velocity with tropinone | Hyoscyamus niger | N-methyl-1-piperidinol + NADP+ | - |
? | |
| 1.1.1.206 | N-propyl-4-piperidinone + NADPH | 78% of the reaction velocity with tropinone | Datura stramonium | N-propylpiperidinol + NADP+ | - |
? | |
| 1.1.1.206 | quinuclidinone + NADPH | 136% of the reaction velocity with tropinone | Hyoscyamus niger | ? | - |
? | |
| 1.1.1.206 | quinuclidinone + NADPH | 80% of the reaction velocity with tropinone | Datura stramonium | ? | - |
? | |
| 1.1.1.206 | tropinone + NADPH + H+ | - |
Datura stramonium | tropine + NADP+ | - |
r | |
| 1.1.1.206 | tropinone + NADPH + H+ | - |
Atropa belladonna | tropine + NADP+ | - |
? | |
| 1.1.1.206 | tropinone + NADPH + H+ | - |
Duboisia myoporoides | tropine + NADP+ | - |
? | |
| 1.1.1.206 | tropinone + NADPH + H+ | - |
Duboisia leichhardtii | tropine + NADP+ | - |
? | |
| 1.1.1.206 | tropinone + NADPH + H+ | - |
Hyoscyamus niger | tropine + NADP+ | - |
r | |
| 1.1.1.206 | tropinone + NADPH + H+ | - |
Hyoscyamus muticus | tropine + NADP+ | - |
? | |
| 1.1.1.206 | tropinone + NADPH + H+ | - |
Scopolia atropoides | tropine + NADP+ | - |
? | |
| 1.1.1.206 | tropinone + NADPH + H+ | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | Datura stramonium | tropine + NADP+ | - |
r | |
| 1.1.1.206 | tropinone + NADPH + H+ | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | Atropa belladonna | tropine + NADP+ | - |
? | |
| 1.1.1.206 | tropinone + NADPH + H+ | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | Duboisia myoporoides | tropine + NADP+ | - |
? | |
| 1.1.1.206 | tropinone + NADPH + H+ | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | Duboisia leichhardtii | tropine + NADP+ | - |
? | |
| 1.1.1.206 | tropinone + NADPH + H+ | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | Hyoscyamus niger | tropine + NADP+ | - |
r | |
| 1.1.1.206 | tropinone + NADPH + H+ | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | Hyoscyamus muticus | tropine + NADP+ | - |
? | |
| 1.1.1.206 | tropinone + NADPH + H+ | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | Scopolia atropoides | tropine + NADP+ | - |
? | |
| 1.1.1.236 | 3-methyl-cyclohexanone + NADPH | - |
Hyoscyamus niger | ? + NADP+ | - |
? | |
| 1.1.1.236 | 4-ethyl-cyclohexanone + NADPH | - |
Hyoscyamus niger | ? + NADP+ | - |
? | |
| 1.1.1.236 | 4-methyl-cyclohexanone + NADPH | - |
Datura stramonium | ? + NADP+ | - |
? | |
| 1.1.1.236 | 4-methyl-cyclohexanone + NADPH | - |
Hyoscyamus niger | ? + NADP+ | - |
? | |
| 1.1.1.236 | 4-tetrahydro-thiopyranone + NADP+ | - |
Datura stramonium | ? | - |
? | |
| 1.1.1.236 | 4-tetrahydro-thiopyranone + NADPH | - |
Atropa belladonna | ? + NADP+ | - |
? | |
| 1.1.1.236 | N-methyl-4-piperidinone + NADPH | - |
Datura stramonium | ? + NADP+ | - |
? | |
| 1.1.1.236 | N-methyl-4-piperidinone + NADPH | - |
Atropa belladonna | ? + NADP+ | - |
? | |
| 1.1.1.236 | N-methyl-4-piperidinone + NADPH | - |
Hyoscyamus niger | ? + NADP+ | - |
? | |
| 1.1.1.236 | N-propyl-4-piperidinone + NADPH | - |
Hyoscyamus niger | ? + NADP+ | - |
? | |
| 1.1.1.236 | tropinone + NADPH + H+ | - |
Solanum tuberosum | pseudotropine + NADP+ | - |
ir | |
| 1.1.1.236 | tropinone + NADPH + H+ | - |
Datura stramonium | pseudotropine + NADP+ | - |
ir | |
| 1.1.1.236 | tropinone + NADPH + H+ | - |
Atropa belladonna | pseudotropine + NADP+ | - |
ir | |
| 1.1.1.236 | tropinone + NADPH + H+ | - |
Hyoscyamus niger | pseudotropine + NADP+ | - |
ir | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.206 | TRI | - |
Datura stramonium |
| 1.1.1.206 | TRI | - |
Atropa belladonna |
| 1.1.1.206 | TRI | - |
Duboisia myoporoides |
| 1.1.1.206 | TRI | - |
Duboisia leichhardtii |
| 1.1.1.206 | TRI | - |
Hyoscyamus niger |
| 1.1.1.206 | TRI | - |
Hyoscyamus muticus |
| 1.1.1.206 | TRI | - |
Scopolia atropoides |
| 1.1.1.206 | tropinone reductase | - |
Datura stramonium |
| 1.1.1.206 | tropinone reductase | - |
Atropa belladonna |
| 1.1.1.206 | tropinone reductase | - |
Duboisia myoporoides |
| 1.1.1.206 | tropinone reductase | - |
Duboisia leichhardtii |
| 1.1.1.206 | tropinone reductase | - |
Hyoscyamus niger |
| 1.1.1.206 | tropinone reductase | - |
Hyoscyamus muticus |
| 1.1.1.206 | tropinone reductase | - |
Scopolia atropoides |
| 1.1.1.236 | pseudotropine forming tropinone reductase | - |
Solanum tuberosum |
| 1.1.1.236 | pseudotropine forming tropinone reductase | - |
Datura stramonium |
| 1.1.1.236 | pseudotropine forming tropinone reductase | - |
Atropa belladonna |
| 1.1.1.236 | pseudotropine forming tropinone reductase | - |
Hyoscyamus niger |
| 1.1.1.236 | TRII | - |
Solanum tuberosum |
| 1.1.1.236 | TRII | - |
Datura stramonium |
| 1.1.1.236 | TRII | - |
Atropa belladonna |
| 1.1.1.236 | TRII | - |
Hyoscyamus niger |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.206 | 25.6 | - |
tropinone | pH 7.0 | Datura stramonium | |
| 1.1.1.236 | 2.73 | - |
tropinone | at pH 7.0 | Datura stramonium | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.206 | 6.1 | - |
reduction reaction | Hyoscyamus niger |
| 1.1.1.206 | 6.4 | - |
reduction reaction | Datura stramonium |
| 1.1.1.206 | 7.6 | - |
oxidation reaction | Hyoscyamus niger |
| 1.1.1.206 | 9.9 | - |
oxidation reaction | Datura stramonium |
| 1.1.1.236 | 5 | - |
- |
Solanum tuberosum |
| 1.1.1.236 | 5.8 | - |
- |
Hyoscyamus niger |
| 1.1.1.236 | 6.25 | - |
- |
Datura stramonium |
| 1.1.1.236 | 6.25 | - |
- |
Atropa belladonna |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.206 | NADPH | - |
Datura stramonium | |
| 1.1.1.206 | NADPH | - |
Atropa belladonna | |
| 1.1.1.206 | NADPH | - |
Duboisia myoporoides | |
| 1.1.1.206 | NADPH | - |
Duboisia leichhardtii | |
| 1.1.1.206 | NADPH | - |
Hyoscyamus niger | |
| 1.1.1.206 | NADPH | - |
Hyoscyamus muticus | |
| 1.1.1.206 | NADPH | - |
Scopolia atropoides | |
| 1.1.1.236 | NADPH | - |
Solanum tuberosum | |
| 1.1.1.236 | NADPH | - |
Datura stramonium | |
| 1.1.1.236 | NADPH | - |
Atropa belladonna | |
| 1.1.1.236 | NADPH | - |
Hyoscyamus niger | |
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