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Literature summary extracted from
- Agmatine deiminase from maize shoots: purification and properties (2001), Phytochemistry, 56, 643-647.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.3.12 | arcaine | competitive inhibitor | Zea mays |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.3.12 | 0.012 | - |
agmatine | - |
Zea mays | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.3.12 | 43500 | - |
1 * 43500 + 1 * 44000, SDS-PAGE | Zea mays |
| 3.5.3.12 | 44000 | - |
1 * 43500 + 1 * 44000, SDS-PAGE | Zea mays |
| 3.5.3.12 | 75000 | - |
gel filtration | Zea mays |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.3.12 | Zea mays | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.3.12 | enzyme purified from shoots grown in the dark by DE52 column chromatography, Sephadex G-100 column chromatography and agmatine-epoxy-Sepharose 6B column chromatography | Zea mays |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.3.12 | shoot | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.3.12 | 0.01 | - |
crude extract of enzyme | Zea mays |
| 3.5.3.12 | 0.015 | - |
enzyme activity of shoots grown for 7 days at 25°C in the dark and light respectively | Zea mays |
| 3.5.3.12 | 32.2 | - |
purified enzyme | Zea mays |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.3.12 | agmatine + H2O | - |
Zea mays | N-carbamoylputrescine + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.3.12 | heterodimer | 1 * 43500 + 1 * 44000, SDS-PAGE | Zea mays |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.3.12 | 4 | - |
purified enzyme stable for two months | Zea mays |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.3.12 | 6.5 | - |
- |
Zea mays |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.3.12 | 6 | - |
purified enzyme shows 85% of maximum activity | Zea mays |
| 3.5.3.12 | 8 | - |
purified enzyme shows 85% of maximum activity | Zea mays |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.3.12 | 0.0033 | - |
arcaine | - |
Zea mays | |
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