BRENDA - Enzyme Database

Agmatine deiminase from maize shoots: purification and properties

Yanagisawa, H.; Phytochemistry 56, 643-647 (2001)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.12
arcaine
competitive inhibitor
Zea mays
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.12
0.012
-
agmatine
-
Zea mays
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.12
43500
-
1 * 43500 + 1 * 44000, SDS-PAGE
Zea mays
3.5.3.12
44000
-
1 * 43500 + 1 * 44000, SDS-PAGE
Zea mays
3.5.3.12
75000
-
gel filtration
Zea mays
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.12
Zea mays
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.12
enzyme purified from shoots grown in the dark by DE52 column chromatography, Sephadex G-100 column chromatography and agmatine-epoxy-Sepharose 6B column chromatography
Zea mays
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.3.12
shoot
-
Zea mays
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.3.12
0.01
-
crude extract of enzyme
Zea mays
3.5.3.12
0.015
-
enzyme activity of shoots grown for 7 days at 25°C in the dark and light respectively
Zea mays
3.5.3.12
32.2
-
purified enzyme
Zea mays
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.12
agmatine + H2O
-
670510
Zea mays
N-carbamoylputrescine + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.3.12
heterodimer
1 * 43500 + 1 * 44000, SDS-PAGE
Zea mays
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.3.12
4
-
purified enzyme stable for two months
Zea mays
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.12
6.5
-
-
Zea mays
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.3.12
6
-
purified enzyme shows 85% of maximum activity
Zea mays
3.5.3.12
8
-
purified enzyme shows 85% of maximum activity
Zea mays
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.3.12
0.0033
-
arcaine
-
Zea mays
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.12
arcaine
competitive inhibitor
Zea mays
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.3.12
0.0033
-
arcaine
-
Zea mays
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.12
0.012
-
agmatine
-
Zea mays
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.12
43500
-
1 * 43500 + 1 * 44000, SDS-PAGE
Zea mays
3.5.3.12
44000
-
1 * 43500 + 1 * 44000, SDS-PAGE
Zea mays
3.5.3.12
75000
-
gel filtration
Zea mays
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.12
enzyme purified from shoots grown in the dark by DE52 column chromatography, Sephadex G-100 column chromatography and agmatine-epoxy-Sepharose 6B column chromatography
Zea mays
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.3.12
shoot
-
Zea mays
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.3.12
0.01
-
crude extract of enzyme
Zea mays
3.5.3.12
0.015
-
enzyme activity of shoots grown for 7 days at 25°C in the dark and light respectively
Zea mays
3.5.3.12
32.2
-
purified enzyme
Zea mays
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.12
agmatine + H2O
-
670510
Zea mays
N-carbamoylputrescine + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.3.12
heterodimer
1 * 43500 + 1 * 44000, SDS-PAGE
Zea mays
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.3.12
4
-
purified enzyme stable for two months
Zea mays
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.12
6.5
-
-
Zea mays
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.3.12
6
-
purified enzyme shows 85% of maximum activity
Zea mays
3.5.3.12
8
-
purified enzyme shows 85% of maximum activity
Zea mays