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Literature summary extracted from

  • Litterer, L.A.; Plaisance, K.L.; Schnurr, J.A.; Storey, K.K.; Jung, H.J.G.; Gronwald, J.W.; Somers, D.A.
    Biosynthesis of UDP-glucuronic acid in developing soybean embryos: Possible role of UDP-sugar pyrophosphorylase (2006), Physiol. Plant., 128, 200-211.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.44 cloned in Escherichia coli Glycine max
2.7.7.64 expression in Escherichia coli Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.44 0.14
-
 1-phospho-alpha-D-glucuronate
-
 Glycine max
2.7.7.44 0.23
-
 alpha-D-glucose 1-phosphate
-
 Glycine max
2.7.7.64 0.14
-
 D-glucuronic acid 1-phosphate pH 8.0 Glycine max
2.7.7.64 0.15
-
 UTP pH 8.0, cosubstrate: glucuronic acid 1-phosphate Glycine max
2.7.7.64 0.19
-
 UTP pH 8.0, cosubstrate: glucose 1-phosphate Glycine max
2.7.7.64 0.23
-
 D-glucose 1-phosphate pH 8.0 Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.44 Mg2+
-
 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.44 71000
-
 SDS-PAGE Glycine max

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.44 Glycine max Q09WE7 Soybean (L.) Merrill
-
2.7.7.64 Glycine max Q09WE7 L. Merr. cv. Lambert
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.44 His-Link affinity resin Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.64 embryo
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.44 additional information
-
 UDP-GlcA PPase activity increases 3.9fold during embryo development (25-45 daf), the activity increases in proportion to the increase in seed fresh weight during embryo development Glycine max
2.7.7.44 0.0019
-
 substrate alpha-D-mannose 1-phosphate Glycine max
2.7.7.44 0.0022
-
 substrate D-glucose 6-phosphate Glycine max
2.7.7.44 0.0034
-
 substrate N-acetyl-alpha-D-glucosamine 1-phosphate Glycine max
2.7.7.44 0.097
-
 substrate alpha-D-glucoronic acid 1-phosphate Glycine max
2.7.7.44 0.11
-
 substrate alpha-D-glucose 1-phosphate Glycine max
2.7.7.44 0.112
-
 substrate alpha-D-galactose 1-phosphate Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.44 UTP + 1-phospho-alpha-D-glucuronate
-
 Glycine max diphosphate + UDP-alpha-D-glucuronate
-
 ?
2.7.7.44 UTP + alpha-D-glucose 1-phosphate
-
 Glycine max ?
-
 ?
2.7.7.64 UTP + D-galactose 1-phosphate 116% of the activity with glucuronic acid 1-phosphate Glycine max diphosphate + UDP-galactose
-
 ?
2.7.7.64 UTP + D-glucose 1-phosphate 114% of the activity with glucuronic acid 1-phosphate Glycine max diphosphate + UDP-glucose
-
 r
2.7.7.64 UTP + D-glucuronic acid 1-phosphate
-
 Glycine max diphosphate + UDP-glucuronic acid
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.7.44 UDP-Glc PPase
-
 Glycine max
2.7.7.44 UDP-glucuronic acid pyrophosphorylase
-
 Glycine max
2.7.7.44 UDP-sugar pyrophosphorylase gene (USP1)
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.44 30
-
 assay at Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.44 8
-
 assay at Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.44 7.5 9 broad pH optimum Glycine max