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Literature summary extracted from

  • Schmuck, E.M.; Board, P.G.; Whitbread, A.K.; Tetlow, N.; Cavanaugh, J.A.; Blackburn, A.C.; Masoumi, A.
    Characterization of the monomethylarsonate reductase and dehydroascorbate reductase activities of Omega class glutathione transferase variants: implications for arsenic metabolism and the age-at-onset of Alzheimers and Parkinsons diseases (2005), Pharmacogenet. Genomics, 15, 493-501.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.8.5.1 medicine significant association with the age-at-onset of Alzheimer's disease and Parkinson's disease Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.5.1 expressed in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.8.5.1 A140D no alteration in specific activity compared to the wild type enzyme Homo sapiens
1.8.5.1 E155 the deletion causes a 2-3fold increase in the specific activity with each substrate and a significant decrease in the enzyme's heat stability, it is also linked to abnormal arsenic excretion patterns Homo sapiens
1.8.5.1 E208K no alteration in specific activity compared to the wild type enzyme Homo sapiens
1.8.5.1 N142D no effect on the specific activity of the enzyme with any substrate Homo sapiens
1.20.4.2 A140D isoform GSTO2, natural polymorphism, no significant alteration in monomethylarsonate reductase activity Homo sapiens
1.20.4.2 DELTAE155 isoform GSTO2, natural polymorphism, 90% increase in monomethylarsonate reductase activity Homo sapiens
1.20.4.2 DELTAE155/E208K isoform GSTO2, natural polymorphism, 100% increase in monomethylarsonate reductase activity, decrease in heat stability Homo sapiens
1.20.4.2 E208K isoform GSTO2, natural polymorphism, slight increase in monomethylarsonate reductase activity, decrease in heat stability Homo sapiens
1.20.4.2 N142D isoform GSTO2, natural polymorphism, no significant alteration in monomethylarsonate reductase activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.5.1 0.5
-
 dehydroascorbate allelic variant D142 of GSTO2-2 Homo sapiens
1.8.5.1 0.5
-
 dehydroascorbate allelic variant N142 of GSTO2-2 Homo sapiens
1.8.5.1 11.4
-
 glutathione allelic variant D142 of GSTO2-2 Homo sapiens
1.8.5.1 11.8
-
 glutathione allelic variant N142 of GSTO2-2 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.1 Homo sapiens P78417
-
-
1.20.4.2 Homo sapiens P78417 isoforms GSTO1, GSTO2 and their natural polymorphisms. Enzyme has additionally thioltransferase, dehydroascorbate reductase and dimethylarsonate reductase activities
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.5.1 Ni-agarose affinity chromatography Homo sapiens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.8.5.1 GSTO2-2, using 8 mM urea Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.5.1 0.13
-
 allelic variant A140/E155/E208 of GSTO1-1, dehydroascorbate as substrate, at 30°C Homo sapiens
1.8.5.1 0.14
-
 allelic variant A140/E155/K208 of GSTO1-1, dehydroascorbate as substrate, at 30°C Homo sapiens
1.8.5.1 0.14
-
 allelic variant D140/E155/E208 of GSTO1-1, dehydroascorbate as substrate, at 30°C Homo sapiens
1.8.5.1 0.21
-
 allelic variant D140/DELTAE155/K208 of GSTO1-1, dehydroascorbate as substrate, at 30°C Homo sapiens
1.8.5.1 0.25
-
 allelic variant A140/DELTAE155/E208 of GSTO1-1, dehydroascorbate as substrate, at 30°C Homo sapiens
1.8.5.1 11.6
-
 allelic variant D142 of GSTO2-2, dehydroascorbate as substrate, at 30°C Homo sapiens
1.8.5.1 13.8
-
 allelic variant N142 of GSTO2-2, dehydroascorbate as substrate, at 30°C Homo sapiens
1.20.4.2 0.27
-
 GSTO1 mutant A140D, 30°C, pH 5.5 Homo sapiens
1.20.4.2 0.33
-
 wild-type GSTO1, 30°C, pH 5.5 Homo sapiens
1.20.4.2 0.39
-
 GSTO1 mutant E208K, 30°C, pH 5.5 Homo sapiens
1.20.4.2 0.42
-
 wild-type GSTO1, 30°C, pH 5.5 Homo sapiens
1.20.4.2 0.44
-
 GSTO2 mutant N142D, 30°C, pH 5.5 Homo sapiens
1.20.4.2 0.65
-
 GSTO1 mutant DELTAE155, 30°C, pH 5.5 Homo sapiens
1.20.4.2 0.67
-
 GSTO1 mutant DELTAE155/E208K, 30°C, pH 5.5 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.1 glutathione + dehydroascorbate
-
 Homo sapiens GSSG + ascorbate
-
 ?
1.8.5.1 glutathione + dehydroascorbate
-
 Homo sapiens glutathione disulfide + ascorbate
-
 r
1.20.4.2 monomethylarsonate + glutathione
-
 Homo sapiens monomethylarsonous acid + oxidized glutathione
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.5.1 dehydroascorbate reductase
-
 Homo sapiens
1.8.5.1 DHA reductase
-
 Homo sapiens
1.8.5.1 DHAR
-
 Homo sapiens
1.8.5.1 GSTO1-1
-
 Homo sapiens
1.8.5.1 GSTO2-2
-
 Homo sapiens
1.20.4.2 GSTO1
-
 Homo sapiens
1.20.4.2 GSTO2
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.5.1 53
-
 at 53°C, both GSTO2-2 isoforms show a similar loss of activity Homo sapiens
1.20.4.2 60
-
 wild-type, 30 min, 60% residual activity. GSTO1 mutant E208K, 30 min, 80% residual activity, GSTO1 mutant DELTAE155/E208K, 30 min, no residual activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.5.1 7.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.5.1 5 8
-
 Homo sapiens