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Literature summary extracted from

  • Youn, B.; Camacho, R.; Moinuddin, S.G.; Lee, C.; Davin, L.B.; Lewis, N.G.; Kang, C.
    Crystal structures and catalytic mechanism of the Arabidopsis cinnamyl alcohol dehydrogenases AtCAD5 and AtCAD4 (2006), Org. Biomol. Chem., 4, 1687-1697.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.195 isozyme CAD4, expression in Escherichia coli Arabidopsis thaliana
1.1.1.195 isozyme CAD5, expression in Escherichia coli Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.195 purified recombinant isozyme CAD5, 4-10 mg/ml apo-protein in 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, and 1 mM DTT, hanging drop vapour diffusion method, at 4°C or at 20°C, 2fold dilution with reservoir solution containing 20% w/v PEG 3350, and 0.2 M trilithium citrate tetrahydrate, pH 8.1 3 days, X-ray diffraction structure determination an analysis at 2.0-2.6 A resolution Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.195 Zn2+ ligand binding structure, Zn2+ is bound in a tetrahedral coordination involving Glu70 Arabidopsis thaliana
1.1.1.195 Zn2+ zinc-type enzyme, ligand binding structure, Zn2+ is bound in a tetrahedral coordination involving Glu70 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.195 cinnamyl aldehyde + NADPH Arabidopsis thaliana
-
 cinnamyl alcohol + NADP+
-
 r
1.1.1.195 coniferyl aldehyde + NADPH Arabidopsis thaliana
-
 coniferyl alcohol + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.195 Arabidopsis thaliana O49482 isozyme CAD5; isozyme CAD5
-
1.1.1.195 Arabidopsis thaliana P48523 isozyme CAD4; isozyme CAD4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.195 recombinant isozyme CAD4 from Escherichia coli Arabidopsis thaliana
1.1.1.195 recombinant isozyme CAD5 from Escherichia coli Arabidopsis thaliana

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.195 cinnamyl alcohol + NADP+ = cinnamaldehyde + NADPH + H+ catalytic reaction mechanism, substrate and cofactor binding structure Arabidopsis thaliana
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.195 5-hydroxyconiferyl aldehyde + NADPH
-
 Arabidopsis thaliana 5-hydroxyconiferyl alcohol + NADP+
-
 r
1.1.1.195 caffeyl aldehyde + NADPH
-
 Arabidopsis thaliana caffeyl alcohol + NADP+
-
 r
1.1.1.195 cinnamyl aldehyde + NADPH
-
 Arabidopsis thaliana cinnamyl alcohol + NADP+
-
 r
1.1.1.195 coniferyl aldehyde + NADPH
-
 Arabidopsis thaliana coniferyl alcohol + NADP+
-
 r
1.1.1.195 coumaryl aldehyde + NADPH + H+
-
 Arabidopsis thaliana coumaryl alcohol + NADP+
-
 r
1.1.1.195 additional information substrate specificity of isozyme CAD4 Arabidopsis thaliana ?
-
 ?
1.1.1.195 additional information substrate specificity of isozyme CAD5 Arabidopsis thaliana ?
-
 ?
1.1.1.195 sinapyl aldehyde + NADPH + H+
-
 Arabidopsis thaliana sinapyl alcohol + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.195 dimer crystal structure Arabidopsis thaliana
1.1.1.195 More structure analysis of isozyme CAD4 by dynamic light scattering Arabidopsis thaliana
1.1.1.195 More structure analysis of isozyme CAD5 by dynamic light scattering Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.1.1.195 CAD
-
 Arabidopsis thaliana
1.1.1.195 cinnamyl alcohol dehydrogenase
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.195 NADP+ cofactor binding site structure determination and analysis Arabidopsis thaliana
1.1.1.195 NADPH cofactor binding site structure determination and analysis Arabidopsis thaliana