EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.195 | isozyme CAD4, expression in Escherichia coli | Arabidopsis thaliana |
1.1.1.195 | isozyme CAD5, expression in Escherichia coli | Arabidopsis thaliana |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.195 | purified recombinant isozyme CAD5, 4-10 mg/ml apo-protein in 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, and 1 mM DTT, hanging drop vapour diffusion method, at 4°C or at 20°C, 2fold dilution with reservoir solution containing 20% w/v PEG 3350, and 0.2 M trilithium citrate tetrahydrate, pH 8.1 3 days, X-ray diffraction structure determination an analysis at 2.0-2.6 A resolution | Arabidopsis thaliana |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.195 | Zn2+ | ligand binding structure, Zn2+ is bound in a tetrahedral coordination involving Glu70 | Arabidopsis thaliana | |
1.1.1.195 | Zn2+ | zinc-type enzyme, ligand binding structure, Zn2+ is bound in a tetrahedral coordination involving Glu70 | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.195 | cinnamyl aldehyde + NADPH | Arabidopsis thaliana | - |
cinnamyl alcohol + NADP+ | - |
r | |
1.1.1.195 | coniferyl aldehyde + NADPH | Arabidopsis thaliana | - |
coniferyl alcohol + NADP+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.195 | Arabidopsis thaliana | O49482 | isozyme CAD5; isozyme CAD5 | - |
1.1.1.195 | Arabidopsis thaliana | P48523 | isozyme CAD4; isozyme CAD4 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.195 | recombinant isozyme CAD4 from Escherichia coli | Arabidopsis thaliana |
1.1.1.195 | recombinant isozyme CAD5 from Escherichia coli | Arabidopsis thaliana |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.195 | cinnamyl alcohol + NADP+ = cinnamaldehyde + NADPH + H+ | catalytic reaction mechanism, substrate and cofactor binding structure | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.195 | 5-hydroxyconiferyl aldehyde + NADPH | - |
Arabidopsis thaliana | 5-hydroxyconiferyl alcohol + NADP+ | - |
r | |
1.1.1.195 | caffeyl aldehyde + NADPH | - |
Arabidopsis thaliana | caffeyl alcohol + NADP+ | - |
r | |
1.1.1.195 | cinnamyl aldehyde + NADPH | - |
Arabidopsis thaliana | cinnamyl alcohol + NADP+ | - |
r | |
1.1.1.195 | coniferyl aldehyde + NADPH | - |
Arabidopsis thaliana | coniferyl alcohol + NADP+ | - |
r | |
1.1.1.195 | coumaryl aldehyde + NADPH + H+ | - |
Arabidopsis thaliana | coumaryl alcohol + NADP+ | - |
r | |
1.1.1.195 | additional information | substrate specificity of isozyme CAD4 | Arabidopsis thaliana | ? | - |
? | |
1.1.1.195 | additional information | substrate specificity of isozyme CAD5 | Arabidopsis thaliana | ? | - |
? | |
1.1.1.195 | sinapyl aldehyde + NADPH + H+ | - |
Arabidopsis thaliana | sinapyl alcohol + NADP+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.195 | dimer | crystal structure | Arabidopsis thaliana |
1.1.1.195 | More | structure analysis of isozyme CAD4 by dynamic light scattering | Arabidopsis thaliana |
1.1.1.195 | More | structure analysis of isozyme CAD5 by dynamic light scattering | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.195 | CAD | - |
Arabidopsis thaliana |
1.1.1.195 | cinnamyl alcohol dehydrogenase | - |
Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.195 | NADP+ | cofactor binding site structure determination and analysis | Arabidopsis thaliana | |
1.1.1.195 | NADPH | cofactor binding site structure determination and analysis | Arabidopsis thaliana |