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Literature summary extracted from

  • Kovari, J.; Imre, T.; Szabo, P.; Vertessy, B.G.
    Mechanistic studies of dUTPases (2004), Nucleosides Nucleotides Nucleic Acids, 23, 1475-1479.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.6.1.23 drug development inhibitor development against the enzyme can be useful in antiviral and anticancer therapy Drosophila melanogaster
3.6.1.23 drug development inhibitor development against the enzyme can be useful in antiviral and anticancer therapy Escherichia coli
3.6.1.23 pharmacology the enzyme is a chemotherapeutic target Drosophila melanogaster
3.6.1.23 pharmacology the enzyme is a chemotherapeutic target Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23
-
 Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.23 additional information the enzyme possesses a specific tryptic cleavage site Arg132-Ile133 20 A far from the active site of the enzyme, the enzyme is protected against tryptic digestion by binding alpha,beta-imino-dUTP which induces an allosteric conformational change within the central threefold channel of the homotrimer Drosophila melanogaster
3.6.1.23 additional information binding of alpha,beta-imino-dUTP does not induce allosteric conformational changes and does not protect the enzyme against tryptic digestion Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 additional information
-
 additional information kinetics of dUTP binding Drosophila melanogaster
3.6.1.23 additional information
-
 additional information kinetics of dUTP binding Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 Mg2+ binding as dUTP-Mg2+ at the C-terminus induces the closure of the C-terminal arm Drosophila melanogaster
3.6.1.23 Mg2+ binding as dUTP-Mg2+ at the C-terminus induces the closure of the C-terminal arm which opens up again after hydrolysis to expel the product Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Drosophila melanogaster the essential enzyme is responsible for preventive DNA repair via exclusion of uracil, overview, lack of negative enzyme regulation leads to thymine-less cell death in cells performing active DNA synthesis dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O Escherichia coli the essential enzyme is responsible for preventive DNA repair via exclusion of uracil, overview, lack of negative enzyme regulation leads to thymine-less cell death in cells performing active DNA synthesis dUMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Drosophila melanogaster
-
-
-
3.6.1.23 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23 recombinant enzyme Drosophila melanogaster

Reaction

EC Number Reaction Comment Organism Reaction ID
3.6.1.23 dUTP + H2O = dUMP + diphosphate structural and catalytic mechanism involving Mg2+ Drosophila melanogaster
a
3.6.1.23 dUTP + H2O = dUMP + diphosphate structural and catalytic mechanism involving Mg2+ Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O the essential enzyme is responsible for preventive DNA repair via exclusion of uracil, overview, lack of negative enzyme regulation leads to thymine-less cell death in cells performing active DNA synthesis Drosophila melanogaster dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O the essential enzyme is responsible for preventive DNA repair via exclusion of uracil, overview, lack of negative enzyme regulation leads to thymine-less cell death in cells performing active DNA synthesis Escherichia coli dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O the enzyme is highly specific for dUTP Drosophila melanogaster dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O the enzyme is highly specific for dUTP Escherichia coli dUMP + diphosphate
-
 ?
3.6.1.23 additional information comparison of the Drosophila melanogaster and the Escherichia coli enzymes Drosophila melanogaster ?
-
 ?
3.6.1.23 additional information comparison of the Drosophila melanogaster and the Escherichia coli enzymes Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.23 trimer each subunit contains an active site Escherichia coli
3.6.1.23 trimer each subunit contains an active site, alpha,beta-imino-dUTP which induces an allosteric conformational change within the central threefold channel of the homotrimer Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 dUTPase
-
 Drosophila melanogaster
3.6.1.23 dUTPase
-
 Escherichia coli