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Literature summary extracted from

  • Shen, Y.; Joachimiak, A.; Rosner, M.R.; Tang, W.J.
    Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism (2006), Nature, 443, 870-874.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.24.56 mutant E111Q in complex with substrates insulin B-chain, amyloid beta-protein, amylin and glucagon. Enzyme forms an enclosed cage just large enough to encapsulate insulin. enclosed substrate undergoes conformational changes to form beta-sheets with two discrete regions of enzyme for degradation Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.56 D426C/K899C 30-40fold increase in activity compared to wild-type Homo sapiens
3.4.24.56 E111Q crystallization data Homo sapiens
3.4.24.56 N184C/Q828C 30-40fold increase in activity compared to wild-type Homo sapiens
3.4.24.56 S132C/E817C 30-40fold increase in activity compared to wild-type Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.56 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.56 amylin + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.56 amyloid beta-protein + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.56 Glucagon + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.56 Insulin B-chain + H2O
-
 Homo sapiens ?
-
 ?