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Literature summary extracted from

  • Aoshima, M.; Igarashi, Y.
    A novel oxalosuccinate-forming enzyme involved in the reductive carboxylation of 2-oxoglutarate in Hydrogenobacter thermophilus TK-6 (2006), Mol. Microbiol., 62, 748-759.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.4.1.7 a fragment containing alpha and beta subunit genes is engineered for heterologous expression in Escherichia coli Hydrogenobacter thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.7 0.788
-
 ATP pH 8.5, 20°C Hydrogenobacter thermophilus
6.4.1.7 1.03
-
 2-oxoglutarate pH 8.5, 20°C Hydrogenobacter thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.4.1.7 ATP + 2-oxoglutarate + HCO3- Hydrogenobacter thermophilus first step of the reductive carboxylation from 2-oxoglutarate to isocitrate. Oxalosuccinate, the product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 1.1.1.41 ADP + phosphate + oxalosuccinate
-
 ?
6.4.1.7 ATP + 2-oxoglutarate + HCO3- Hydrogenobacter thermophilus TK-6 / IAM 12695 first step of the reductive carboxylation from 2-oxoglutarate to isocitrate. Oxalosuccinate, the product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 1.1.1.41 ADP + phosphate + oxalosuccinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.7 Hydrogenobacter thermophilus
-
 TK-6
-
6.4.1.7 Hydrogenobacter thermophilus TK-6 / IAM 12695
-
 TK-6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.7 recombinant Hydrogenobacter thermophilus

Storage Stability

EC Number Storage Stability Organism
6.4.1.7 4°C, stable for at least 1 month Hydrogenobacter thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.7 ATP + 2-oxoglutarate + HCO3-
-
 Hydrogenobacter thermophilus ADP + phosphate + oxalosuccinate
-
 ?
6.4.1.7 ATP + 2-oxoglutarate + HCO3- first step of the reductive carboxylation from 2-oxoglutarate to isocitrate. Oxalosuccinate, the product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 1.1.1.41 Hydrogenobacter thermophilus ADP + phosphate + oxalosuccinate
-
 ?
6.4.1.7 ATP + 2-oxoglutarate + HCO3-
-
 Hydrogenobacter thermophilus TK-6 / IAM 12695 ADP + phosphate + oxalosuccinate
-
 ?
6.4.1.7 ATP + 2-oxoglutarate + HCO3- first step of the reductive carboxylation from 2-oxoglutarate to isocitrate. Oxalosuccinate, the product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 1.1.1.41 Hydrogenobacter thermophilus TK-6 / IAM 12695 ADP + phosphate + oxalosuccinate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.4.1.7 70 80
-
 Hydrogenobacter thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.4.1.7 90
-
 inactivation above Hydrogenobacter thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.4.1.7 30.6
-
 2-oxoglutarate pH 8.5, 20°C Hydrogenobacter thermophilus
6.4.1.7 31.7
-
 ATP pH 8.5, 20°C Hydrogenobacter thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.7 8
-
-
 Hydrogenobacter thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.7 biotin enzyme contains biotin Hydrogenobacter thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.4.1.7 Hydrogenobacter thermophilus isoelectric focusing
-
 4.9