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Literature summary extracted from

  • Yoneyama, N.; Morimoto, H.; Ye, C.X.; Ashihara, H.; Mizuno, K.; Kato, M.
    Substrate specificity of N-methyltransferase involved in purine alkaloids synthesis is dependent upon one amino acid residue of the enzyme (2006), Mol. Genet. Genomics, 275, 125-135.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.160 DNA and amino acid sequence determination and analysis, expression of wild-type and chimeric mutant enzymes in Escherichia coli strain BL21(DE3), phylogenetic analysis Theobroma cacao
2.1.1.160 DNA and amino acid sequence determination and analysis, expression of wild-type and chimeric mutant enzymes in Escherichia coli strain BL21(DE3), phylogenetic analysis Camellia ptilophylla
2.1.1.160 isozyme 1, DNA and amino acid sequence determination and analysis, expression of wild-type and chimeric mutant enzymes in Escherichia coli strain BL21(DE3), phylogenetic analysis Camellia irrawadiensis

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.160 additional information construction and substrate specificity determination of chimeric mutant enzymes composed of theobromine synthase and inactive caffeine synthase, EC 2.1.1.160, overview Theobroma cacao
2.1.1.160 additional information construction and substrate specificity determination of chimeric mutant enzymes composed of theobromine synthase and inactive caffeine synthase, EC 2.1.1.160, overview Camellia irrawadiensis
2.1.1.160 additional information construction and substrate specificity determination of chimeric mutant enzymes composed of theobromine synthase and inactive caffeine synthase, EC 2.1.1.160, overview Camellia ptilophylla

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.160 S-adenosyl-L-methionine + 1,7-dimethylxanthine Theobroma cacao i.e. paraxanthine, alternative route in caffeine biosynthesis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
2.1.1.160 S-adenosyl-L-methionine + 1,7-dimethylxanthine Camellia irrawadiensis i.e. paraxanthine, alternative route in caffeine biosynthesis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
2.1.1.160 S-adenosyl-L-methionine + 1,7-dimethylxanthine Camellia ptilophylla i.e. paraxanthine, alternative route in caffeine biosynthesis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.160 Camellia irrawadiensis Q2HXL9 isozyme 1
-
2.1.1.160 Camellia ptilophylla Q2HXI6
-
-
2.1.1.160 Theobroma cacao Q2HXL8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.160 additional information substrate specificity, overview Theobroma cacao ?
-
 ?
2.1.1.160 additional information substrate specificity, overview Camellia irrawadiensis ?
-
 ?
2.1.1.160 additional information substrate specificity, overview Camellia ptilophylla ?
-
 ?
2.1.1.160 S-adenosyl-L-methionine + 1,7-dimethylxanthine i.e. paraxanthine Camellia irrawadiensis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
2.1.1.160 S-adenosyl-L-methionine + 1,7-dimethylxanthine i.e. paraxanthine Camellia ptilophylla S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
2.1.1.160 S-adenosyl-L-methionine + 1,7-dimethylxanthine i.e. paraxanthine, alternative route in caffeine biosynthesis Theobroma cacao S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
2.1.1.160 S-adenosyl-L-methionine + 1,7-dimethylxanthine i.e. paraxanthine, alternative route in caffeine biosynthesis Camellia irrawadiensis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
2.1.1.160 S-adenosyl-L-methionine + 1,7-dimethylxanthine i.e. paraxanthine, alternative route in caffeine biosynthesis Camellia ptilophylla S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
2.1.1.160 S-adenosyl-L-methionine + 1,7-dimethylxanthine i.e. paraxanthine, preferred substrate Theobroma cacao S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?